Panel B.比较这两个试剂盒在总RNA以 50 pg起始制备文库的转录表达水平 (见Panel A), SMART-Seq Stranded Kit (Pearson =0.97)的再现性高于Pico v2 (pearson =0.92)。 图4. 不同起始量样本的高重现性 利用FACS分离出A375细胞,分别以1-1,000个细胞起始使用SMART-Seq Stranded Kit制备测序文库。其中,以5-1...
Clontech, a wholly-owned subsidiary of Takara Bio of Japan, said it continues to develop related technologies, such as the recently launched DNA SMART kit for ChIP-seq library construction from low-input double-stranded and single-stranded DNA templates....
(Section V.A) • One dedicated thermal cycler used only for double-stranded cDNA amplification by PCR (Section V.B) • High Sensitivity DNA Kit (Agilent, Cat. No. 5067-4626; Section V.D) • Nuclease-free thin-wall PCR tubes or strips (0.2-ml PCR 8-tube strip; USA Scientific, ...
Library complexity is preserved from as little as 500 pg of double-stranded or single-stranded input DNA. The kit utilizes Clontech’s DNA SMART technology; sequencing libraries are generated without the need for enzymatic cleanup or adapter ligations. All of the necessary reagents for producing ...
Then, we extracted a 20-bp window of genome sequence upstream of the read start position on the positive strand (+stranded mappings) or downstream of the read start position+read length on the negative strand (−stranded mappings) using the BSgenome package (version 1.62.0, human hg38). ...
LAST-seq: single-cell RNA sequencing by direct amplification of single-stranded RNA without prior reverse transcription and second-strand synthesis Jun Lyu Chongyi Chen Genome Biology (2023) De novo identification of expressed cancer somatic mutations from single-cell RNA sequencing data Tianyun Zh...
LAST-seq: single-cell RNA sequencing by direct amplification of single-stranded RNA without prior reverse transcription and second-strand synthesis Jun Lyu Chongyi Chen Genome Biology (2023) De novo identification of expressed cancer somatic mutations from single-cell RNA sequencing data Tianyun Zh...