从测序数据随机抽取250万paired-end reads进行生物学信息分析。 Panel B.比较这两个试剂盒在总RNA以 50 pg起始制备文库的转录表达水平 (见Panel A), SMART-Seq Stranded Kit (Pearson =0.97)的再现性高于Pico v2 (pearson =0.92)。 图4. 不同起始量样本的高重现性 利用FACS分离出A375细胞,分别以1-1,000个...
Clontech, a wholly-owned subsidiary of Takara Bio of Japan, said it continues to develop related technologies, such as the recently launched DNA SMART kit for ChIP-seq library construction from low-input double-stranded and single-stranded DNA templates....
Sequencing User Manual Adaptations to SMART technology for next-generation sequencing (NGS) were incorporated into the first- generation of our kit for ultra-low input mRNA-seq (the SMARTer® Ultra Low RNA Kit for Illumina Sequencing) and published as the SMART-Seq method (Ramsköld et al....
The DNA SMART ChIP-Seq Kit generates indexed ChIP-seq libraries suitable for NGS on Illumina® sequencing platforms. Library complexity is preserved from as little as 500 pg of double-stranded or single-stranded input DNA. The kit utilizes Clontech’s DNA SMART technology; sequencing libraries ...
Current single-cell RNA sequencing (scRNA-seq) methods with high cellular throughputs sacrifice full-transcript coverage and often sensitivity. Here we describe Smart-seq3xpress, which miniaturizes and streamlines the Smart-seq3 protocol to substantially reduce reagent use and increase cellular throughpu...
Smart-seq2 improves yield and length in single cell-derived cDNA libraries and uses off-the-shelf reagents. Single-cell gene expression analyses hold promise for characterizing cellular heterogeneity, but current methods compromise on either the coverage
LAST-seq: single-cell RNA sequencing by direct amplification of single-stranded RNA without prior reverse transcription and second-strand synthesis Jun Lyu Chongyi Chen Genome Biology (2023) De novo identification of expressed cancer somatic mutations from single-cell RNA sequencing data Tianyun Zh...
Libraries were mixed with an ϕX bacteriophage genome library to introduce diversity and optimize the sequencing run performance and sequenced using the Illumina MiSeq v2 Reagent Kit. The pair-end runs were initiated for Illunima’s sequencing by synthesis technology, including clustering, paired-...
Libraries were mixed with an φX bacteriophage genome library to introduce diversity and optimize the sequencing run perfor- mance and sequenced using the Illumina MiSeq v2 Reagent Kit. The pair- end runs were initiated for Illunima's sequencing by synthesis technology, including clustering, paired...
MiSeq v2 Reagent Kit. The pair-end runs were initiated for Illunima’s sequencing by synthesis technology, including clustering, paired-end preparation, barcode sequencing and analysis. After completion of the run, base calling was performed on data, sequences were de-multiplexed and ϕX reads...