Panel B.比较这两个试剂盒在总RNA以 50 pg起始制备文库的转录表达水平 (见Panel A), SMART-Seq Stranded Kit (Pearson =0.97)的再现性高于Pico v2 (pearson =0.92)。 图4. 不同起始量样本的高重现性 利用FACS分离出A375细胞,分别以1-1,000个细胞起始使用SMART-Seq Stranded Kit制备测序文库。其中,以5-1...
SMARTer Stranded RNA-Seq Kit 提供了一种构建Illumina链特异性测序文库的解决方案,并且链特异性识别准确率>99%,可以从低至100 pg至100 ng的RNA起始获得可靠数据。SMARTer Stranded RNA-Seq Kit 采用随机引物法适用于原核或真核RNA,推荐使用去除rRNA或poly(A)富集RNA样品。本试剂采用改良的核心SMART技术,可以生成完整...
除细胞样本外,FFPE和游离RNA样本也是融合基因RNA-Seq项目中具有应用潜力的样本类型。这两种类型的样本常常存在完整性不足的问题,并且在样本品质和提取量方面也有各自的不足,为后续的NGS分析工作增加了难度。 Takara针对性推出了SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian。优化的文库构建流程...
Clontech, a wholly-owned subsidiary of Takara Bio of Japan, said it continues to develop related technologies, such as the recently launched DNA SMART kit for ChIP-seq library construction from low-input double-stranded and single-stranded DNA templates....
方案一:SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian(Code No.634411-634414) 在使用鼻拭子、咽拭子、肺泡灌洗液等临床样本进行病毒NGS分析时,往往会遇到病毒含量少、样本背景复杂、建库过程PCR扩增不均一等问题,给正确分析病毒基因组信息造成困难。
以 Jurkat 单细胞(6个重复)为样本制备文库,分别采用 NEBNext单细胞/超低起始量 RNA 文库制备试剂盒、SMART-Seq v4 Ultra Low Input RNA 试剂盒(Clontech #634891)及Nextera XT DNA Library Prep 试剂盒(Illumina #FC-131-1096)制备文库。文库通过 Illumina NextSeq 500(2 x 76 bp)双端测序。TPM = 每百万...
(including microRNA), for inputs ranging from 1 ng–2 μg. By incorporating features of the SMARTer Stranded RNA-Seq kits, including our proprietary SMART (SwitchingMechanismat the 5' end ofRNATemplate) technology, and primers that include locked nucleic acids (LNAs), this kit ...