Sequencing of miRNAs isolated from exosomes has great potential to identify novel disease biomarkers, but exosomes have low amount of RNA, hindering adequate analysis and quantification. Here, we have assessed several steps in developing an optimized small RNA (sRNA) library preparation protocol for ...
et al. Zcchc11-dependent uridylation of microRNA directs cytokine expression. Nature cell biology 11, 1157–1163 (2009). Article CAS PubMed PubMed Central Google Scholar Koppers-Lalic, D. et al. Nontemplated nucleotide additions distinguish the small RNA composition in cells from exosomes. ...
Methods for the extraction and RNA profiling of exosomes AIM:To develop protocols for isolation of exosomes and characterization of their RNA content.METHODS:Exosomes were extracted from He La cell culture media ... E Zeringer,M Li,T Barta,... - 《World Journal of Methodology》 被引量: 55...
A simple injection of the genetic circuits leads to the automatic production and self-assembly of siRNAs into circulating exosomes, which are spontaneously transferred through the naturally existing small RNA transportation machinery to the target cells. Our strategy avoids the complicated procedures, high...
Small Cytoplasmic RNA In subject area: Pharmacology, Toxicology and Pharmaceutical Science About this pageAdd to MendeleySet alert Also in subject areas: Biochemistry, Genetics and Molecular Biology Medicine and Dentistry NeuroscienceShow moreDiscover other topics ...
Small RNA deep sequencing reveals a distinct miRNA signature released in exosomes from prion-infected neuronal cells Nucleic Acids Res., 40 (2012), pp. 10937-10949 CrossrefView in ScopusGoogle Scholar Calder et al., 2013 A. Calder, I. Roth-Albin, S. Bhatia, C. Pilquil, J.H. Lee, M...
METHODS OF USING SMALL RNA FROM BODILY FLUIDS FOR DIAGNOSIS AND MONITORING OF NEURODEGENERATIVE DISEASES 来自 百度文库 喜欢 0 阅读量: 13 申请(专利)号: US14146275 申请日期: 20140102 公开/公告号: US20140357507A1 公开/公告日期: 20141204 申请(专利权)人: DiamiR LLC 发明人:...
In this study, we used different EV isolation and purification methods to characterize the plasma-derived EV miRNAs from non-smokers, smokers and patients with COPD. A small RNA sequencing (RNA-seq) approach was adapted to identify novel circulating EV miRNAs. We found that plasma-derived EVs ...
Small RNA pathways play key and diverse regulatory roles in C. elegans, but our understanding of their conservation and contributions in other nematodes is limited. We analyzed small RNA pathways in the divergent parasitic nematode Ascaris. Ascaris has t
et al. Small RNA sequencing across diverse biofluids identifies optimal methods for exRNA isolation. Cell 177, 446–462 (2019). Article CAS PubMed PubMed Central Google Scholar Thery, C., Amigorena, S., Raposo, G. & Clayton, A. Isolation and characterization of exosomes from cell culture ...