Efficient RNA extraction from exosomes with SBI’s most flexible SeraMir kit When you want to extract RNA from already isolated exosomes, choose the SeraMir Exosome RNA Column Purification Kit. With only SBI’s highly efficient SeraMir RNA purification columns and reagents, you can keep your exosome...
Extraction of exosomes can be done the day of sample collection or samples can be frozen at −80 °C until use (see Note 3). 2.1 Exosome Isolation 2.1.1 Exosome Isolation 1. Magnetic beads, Anti-EpCAM antibodies coupled to magnetic by Immunoisolation microbeads (DynaBeads, Invitrogen). ...
Exosomes from non-animal organisms remain underestimated, and our understanding of them is still expansible, albeit animal-derived exosomes are moving increasingly into interest areas of research [23]. This is due to the lack of a practicable and easy visualization, purification, and isolation proced...
Versatile plasma/serum input ranging from 2 mL to 5 mL; No phenol extractions; No carrier RNA; Bind and elute all RNA irrespective of size or GC content, without bias; Concentrate circulating RNA and exosomal RNA into a flexible elution volume ranging from 50 µL to 100 µL; Purify ...
从牦牛乳中提取ＲＮＡ_用于乳腺特异蛋白编码基因的扩增
The method comprises the separation of particles termed exosomes from the blood plasma of an individual infected with hepatitis C virus (HCV) and the extraction or RNA from these exosome particles.SERGIO ABRIGNANIPIERO PILERI
©SpringerScience+BusinessMediaNewYork2016 Chapter11 ProtocolsforExosomeIsolationandRNAProfiling FernandoMarqués-GarcíaandMaríaIsidoro-García Abstract Exosomesaresmallextracellularvesiclesofmultivesicularbodiesderivedfromthecellendosome.Molecules haveacharacteristiccompositionbothinthemembraneandinthecarriedload.These...
RNA extraction and quantitative real-time polymerase chain reaction Total RNA was extracted from tissues or cultured cells using TRIzol reagent (Invitrogen). Total RNA in serum was purified using miRNeasy Serum/Plasma kit according to the manufacturer’s instructions (Qiagen). Exosomal RNA was isolated...
★操作安全性提高:使用 RNA Extraction Agent 替代了氯仿 ★适用于从细胞、组织、新鲜血液、外泌体中提取 miRNA 和 Total RNA ★通过调整加入水相中无水乙醇的用量,可获得: 1. RNA 离心柱吸附大分子量 RNA(28S rRNA, 18S rRNA, mRNA) 后,将流出液 ( 包含小于 200 nt 的 RNAs, 如 miRNA,siRNA, shRNA,...
The exosome extraction reagent was used to precipitate the exosome from serum, which were then centrifuged at 1,500 × gfor 10 min at 4°C to remove the supernatant. The exosome pellet was resuspended in 10 mM PBS in four times the volume of serum. A copper mesh was placed on a ...