许金金China Medical Abstracts (Internal Medicine)Extraction andidentification of exosomes from drug-resistant breast cancer cells and theirpotential role in cell-to-cell drug-resistance transfer.]. Xu J,Li W,Zhong S,Li X,Chen Z,Hu Q,Tang J,Zhao J. Chinese Journal of Oncology . 2014...
Wu, X.et al.(2019). Exosomes Extraction and Identification. In: Batra, J., Srinivasan, S. (eds) Theranostics. Methods in Molecular Biology, vol 2054. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9769-5_4 Download citation ...
Extraction and identification of exosomes from human USCs The USC medium was collected and centrifuged at 300 × g (4 °C, 10 min). The supernatant was then collected and centrifuged at 2,000 × g (first cycle, 4 °C, 10 min) and 10,000 × g (second cycle, 4 °C, 30 min). Th...
Exosome extraction and identification Exosomes were obtained via rapid centrifugation. First, the cell culture supernatants of hUCMSCs (cells passaged three – five times) were collected and centrifuged at 2,000 × g for 10 min and dead cells were removed. For the remaining supernatant, the cent...
Exosome Extraction and Identification Briefly, cell growth medium was replaced with medium (without FBS) when the confluence reached 80%. Next, the supernatant was centrifuged (10 min at 300 × g, 15 min at 2000 × g and 30 min at 10,000 × g). Ultracentrifugation (70 min at 120,000 ...
Extraction and identification of ADSC-exos and MSGMSC-exos The 3–6 passages of ADSCs and MSGMSCs were proliferated to approximately 70 percent of confluent and then cultured in MSC NutriStem® XF Medium (05–200-1A; Biological Industry, Israel) for 24 h to obtain FBS-free conditioned medi...
However, the clinical application of exosomes remains subject to limitations. The methods used to isolate, purify, and identify exosomes in the laboratory cannot be used in clinical research because of inconvenience. Firstly, the extraction process of exosomes is relatively complicated, requiring multiple...
Extraction and identification of exosomes derived from HUC-MSCs A 10cm umbilical cord was taken from a sterile operating table, drained the blood and rinsed the surface blood with sterile saline. Put it into a 50 mL centrifuge tube containing 25 mL sterile PBS (Gibco, NY, USA, C2001500BT)...
In studies of exosomes and their practical usage, the extraction and identification methods of exosomes are the research bases. First, they must be separated and purified from both biological fluids and in vitro cell cultures. The choice of exosome isolation techniques noticeably affects the exosome ...
Exosomes are double-layered lipid membranous nanovesicles that are endosomal in origin and secreted by almost all cells. They are 30–130 nm in size and contain various molecular signatures such as miRNAs, mRNAs, DNA, lipids, and proteins. Due to their highly heterogeneous content, exosomes have...