Recently, there is a growing interest of research focused on studying the exosome functions and to understand ways to use them for therapeutic applications in a wide variety of disorders, such as cancer, cardiovascular, neurodegenerative, and musculoskeletal diseases. Recently, a number of techniques ...
Extraction and identification of synovial tissue-derived exosomes by different separation techniques. J. Orthop. Surg. Res.15, 97 (2020). PubMed PubMed Central Google Scholar Kamerkar, S. et al. Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer. Nature546, 498–...
Exosome extraction and identification Exosomes were obtained via rapid centrifugation. First, the cell culture supernatants of hUCMSCs (cells passaged three – five times) were collected and centrifuged at 2,000 × g for 10 min and dead cells were removed. For the remaining supernatant, the cent...
目的· 从多囊卵巢综合征患者卵泡液中分离提取外泌体并进行鉴定,明确卵泡液中外泌体的存在;从外泌体中分离提取 miRNAs 并检测其表达量。方法 · 利用膜亲和法提取卵泡液中外泌体,透射电镜观察大小和形态;利用流式细胞仪检测外泌体蛋白标 志物CD63 和 CD81 的表达;提取
In studies of exosomes and their practical usage, the extraction and identification methods of exosomes are the research bases. First, they must be separated and purified from both biological fluids and in vitro cell cultures. The choice of exosome isolation techniques noticeably affects the exosome ...
At the end of the experiment (3, 7, or 28 days after MI or sham operation), the hearts were collected and cut in half; one half was used for histological analyses, while the other half was used for protein extraction. Echocardiography The primary outcome of this study will be cardiac ...
1C). In a subsequent engagement involving the incubation of Exo-MSCdil and Exo-Yoda1dil with BMSCs for a duration of 4 h, positive staining for Dil was showed in the BMSCs, thus affirming the successful endocytic uptake of the exosomes (Fig. 1D). Fig. 1 Identification of Exosomes (A...
components within exosomes can contribute to tailoring the therapeutic efficacy of exosomes or developing exosome editing strategies to achieve uniform therapeutic safety and efficacy [175]. In addition, standardized procedures for culture, extraction, isolation, and identification need to be established to...
Extraction and identification of mouse islet microvascular endothelial cells (MS-1)-derived exosomes DOI: 10.6040/j.issn.1671-7554.0.2014.348 肖芳, 蒋卫东, 王晓红, 秦爱琼, 韩敏, 陈丽XIAO Fang, JIANG Weidong, WANG Xiaohong, QIN Aiqiong, HAN Min, CHEN Li Keywords: 小鼠胰岛内皮细胞,提取,离心,...
Exosome Extraction and Identification Briefly, cell growth medium was replaced with medium (without FBS) when the confluence reached 80%. Next, the supernatant was centrifuged (10 min at 300 × g, 15 min at 2000 × g and 30 min at 10,000 × g). Ultracentrifugation (70 min at 120,000 ...