Exosome DNA Extraction Kits:基于化学沉淀的原理,一步法快速分离提取外泌体,随后利用经过优化的DNA提取配方,提取出高纯度的外泌体DNA,可用于下游分析系列包括:PCR, RT-PCR, qRT-PCR 以及microarrays。(1小时45分钟完成实验) 图2:Exosome DNA Extraction Kits实验方案展示 ...
提取的外泌体可应 用于ELISA,FACS分析,裂解后可做Western Blot检测,但不建议用于外泌体标记和蛋白质质谱分析;提取的外泌体可用于外泌体相关核酸研究,如miRNAs,siRNAs,mRNAs和DNA,适用于Q-PCR,microarray,NGS测序等分析。 操作说明 产品数据 网站资料更新中......
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and infectious diseases. They are crucial in screening for cancer through exosome isolation kits and extraction procedures that analyze body fluids for biomarkers. Challenges include lack of awareness and stringent regulations, yet collaborations withgovernment associationsand theVAHealthcare system enhance ad...
PEG-based exosomes extraction kits have been developed as ExoQuick (Systems Biosciences) and Total Exosome Isolation Kit (Thermo Fisher Scientific) Lack of specificity, selectivity and low purity (other non-exosomal contaminants as proteins and polymeric materials may co-precipitate) (Soares Martins et...
目前该公司的产品多达700余种,提供一流的样品制备试剂盒,应用于基因组和蛋白组等不同的领域。其产品主要覆盖包括各种标本类型的DNA,RNA和蛋白质的纯化富集,从活检样本(包括尿液,血浆,血清和血液)中纯化RNA和DNA,全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。
(f) The nuclear extracts of cells were assayed for the DNA-binding activity of p65/c-Rel (NF-κB). Data are presented as the mean±s.e.m. (n=3), and Tukey’spost hoctest was applied to significant group effects (**P<0.01) identified by ANOVA. Scale bars, 20 μm. NS, not ...
Extraction of RNA and conducting of PCR assay TRIzol reagent (Invitrogen, Waltham, MA, USA) was used to extract total RNA from the tissues and cell lines, following the manufacturer’s instructions. To generate complementary DNA (cDNA), random primers and the FastKing RT Kits (TIANGEN Biotech...
目前该公司的产品多达700余种,提供一流的样品制备试剂盒,应用于基因组和蛋白组等不同的领域。其产品主要覆盖包括各种标本类型的DNA,RNA和蛋白质的纯化富集,从活检样本(包括尿液,血浆,血清和血液)中纯化RNA和DNA,全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。
1a). This ZFP is capable of binding to the NF-kB doublet binding site within the LTR promoter of HIV-1 and was fused to a DNA methyl transferase 3 domain (3 A) to generate (ZD3A). Transfection of ZD3A into chronically HIV-1-infected Jurkat cells (CHI-Ju) led to significantly ...