38 4 Product Information Product Description Introduction The Total Exosome RNA and Protein Isolation Kit is designed for isolation of RNA and protein from a single enriched exosome preparation. Resuspended exosomes are divided, with one portion being used directly for common protein analysis applications...
The Total Exosome RNA and Protein Isolation Kit is designed for the isolation of both RNA and protein from pre-isolated or enriched exosomes (not supplied). It is suitable for studies of RNA expression (specifically miRNA), processing, or function. The kit allows recovery of both RNA and pro...
Isolation of exosomes for subsequent mRNA, MicroRNA, and protein profiling. Methods Mol. Biol. 784 181–195. 10.1007/978-1-61779-289-2_13 [ Cross Ref ]Rani S., O’Brien K., Kelleher F. C., et al. Isolation of exosomes for subsequent mRNA, MicroRNA, and protein profiling. Methods ...
Exosomes were originally described as small vesicles released from reticulocytes during the maturation process. These 40-200 nm microvesicles were hypothesized to be a mechanism for the removal of membrane proteins in lieu of intracellular degradation by Harding et al. (1984) and Johnstone et al. ...
exosomes in serum samples from cancer patients53. In addition to digital counting of exosomes, the DigitISA technique allows us to extract the number of CD63 molecules present on the exosome surface (i.e., expression level). This is done by determining the individual burst intensity of each ...
Recent advances in extracellular vesicle (EVs) detection and isolation methods have led to the development of novel therapeutic modalities. Among different types of EVs, exosomes (Exos) can transfer different signaling biomolecules and exhibit several superior features compared to whole-cell-based therapie...
Exosome isolation Exosomes were isolated by differential centrifugation as described previously.35For further exosome purification, pellets were resuspended in 4 mL 2.55 M sucrose, 20 mM HEPES (N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid)/NaOH, pH 7.4. A linear sucrose gradient (2.25 to 0...
M., Kruh-Garcia, N. A. Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum. J. Vis. Exp. (134), e57467, doi:10.3791/57467 (2018). Download Citation Reprints and Permissions JoVE Privacy ...
8B). Following infection of replication-incompetent Prnp-/- cells with exosomes from chronically infected S7 cells, 6D11-positive spots were detected intracellularly 1, 2 and 3 days after infection (Supplementary Fig. 8C). Remarkably, 5B2-positive FL-PrPd was not detected in susceptible S7 ...
Microfluidic device (ExoChip) for on-chip isolation, quantification and characterization of circulating exosomes. Lab Chip 14, 1891–1900. https://doi.org/10.1039/c4lc00136b (2014). Article CAS PubMed PubMed Central Google Scholar Tian, T., Wang, Y., Wang, H., Zhu, Z. & Xiao, Z. ...