Exosome isolationDisease diagnosticsOn-chip analysisExosomes are nanoscale membrane vesicles (similar to 30-150 nm) actively released by most types of cells, which has great potential for non-invasive disease diagnostics. Current isolation techniques such as ultracentrifugation face a number of drawbacks ...
5]. Exosomes are one of the extracellular vesicles (EVs) secreted by mother cells in the extracellular environment and are widely distributed in body fluids, which have a bilayered phospholipid membrane structure [6], exosomes contain large amounts of biologically active proteins, lipids, nucleic ac...
major histocompatibility complex (MHC), heat shock proteins (Hsp), transcription factors, cytoskeletal components, nucleic acids (genomic and mitochondrial DNA and RNA), and lipids are common loaders for exosomes (Fig.1B) [7]. Among them, the family of four transmembrane proteins and heat shock ...
Effective isolation of exosomes with polyethylene glycol from cell culture supernatant for in-depth proteome profiling. Analyst. 2016;141:4640–6. 42. Niu Z, Pang RTK, Liu W, Li Q, Cheng R, Yeung WSB. Polymer-based precipitation preserves biological activities of extracellular vesicles from an ...
Here, we summarize the current evidence about the mechanisms underlying the recognition and sequestration of therapeutic exosomes by phagocytic cells. Also, we propose different strategies to generate 'invisible' exosomes for the immune system, through the incorporation of different anti-phagocytic ...
The liver is the most important metabolic organ in the body. While mouse models and cell lines have further deepened our understanding of liver biology and related diseases, they are flawed in replicating key aspects of human liver tissue, particularly i
The active role of exosomes was previously proved on blood vessels development and progression [91]. Recent evidence suggests the existence of specific miRNAs inside exosomes with the angiogenic potential in the target cells by the induction of VEGF and different signaling pathways [92, 93]. In ...
Exosomes, on the other hand, can be used as vehicles for the delivery of therapeutic agents to target cells. Understanding the complex interplay between non-coding RNA, exosomes, and tumor-associated macrophages will provide valuable insights for the development of more effective cancer treatments. ...
lateral wall transplantation of BMSCs [103]. Therefore, BMSCs have the potential to serve as seed cells for both differentiation into SGNs and maintenance of inner ear homeostasis. However, there are still some inherent limitations to the source of BMSCs, such as a painful isolation process ...
tumor antigens along with adjuvants to DCs and promoting their migration to LNs. Then, CpG-SAV-exo successfully induced immunostimulatory signals to inhibit tumor growth more significantly than the simple administration of exosomes and CpG DNA in tumor-bearing mice, indicating that the tumor antigen...