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外泌体RNA提取试剂盒(基于完整的外泌体)|Exosomal RNA Isolation Kit (for use with Urine Exosome Purification Kits or Plasma/Serum Exosome 价格电议 起批量≥1 preps 最小起订1preps 供货总量50preps 发货地址湖北省武汉市 建议售价￥11/preps 更新日期2024年05月20日 ...
Whole transcriptome RNA sequencing of exosomes and bioinformatics analysis Total RNA was isolated from exosomes derived from SW480 cells that had been induced (termed the EMT-exo group) or not induced by TGF-β (termed the non-EMT-exo group) using an Exosomal RNA Isolation Kit (NGB-58,000,...
Isolation of exosomal RNA from urine using the Qiagen miRNeasy Micro kitThis protocol describes how to isolate exosomal RNA from urine by using the Qiagen ExoRNeasy maxi kit.doi:10.1038/protex.2017.083Roger P. AlexanderSrimeenakshi Srinivasan
The exosomal RNA was prepared via using Exosome Purifica- tion and RNA Isolation kit (AmyJet Scientific, Wuhan, China). circRNA was purified via digesting the linear RNA using RNase R (Geneseed, Guangzhou, China). One μg RNA was used to complementary DNA synthetization using the miRNA ...
This protocol describes how to isolate exosomal RNA from urine by using the Exoquick Seramir tissue culture kit.doi:10.1038/protex.2017.080Roger P. AlexanderSrimeenakshi SrinivasanLouise C. LaurentProtocol Exchange
For qRT‒PCR, total RNA was extracted from MSCs, MDSCs, and glioma cells using TRIzol in accordance with the manufacturer’s protocol. Exosomal RNA was extracted using the SeraMir™ Exosome RNA Extraction Kit (System Biosciences, USA) following the manufacturer’s protocol. A ReverTra Ace qP...
RNA isolation, PCR and western blotting TRIzol reagent was utilized to extract total RNA from cells, patient serum and plasma exosomes, with cel-miR- 39 added to serum or exosome solutions before chloro- form. Cel-miR-39 was used as the external reference for the PCR analysis of miRNA in ...
RNA isolation and quantitative RT-PCR (qRT-PCR). Total RNA from cells was extracted using the acid guanidinium thiocyanate-phenol-chloroform extraction method, and total RNA from exosomes was extracted using a Total Exosome RNA and Protein Isolation kit (Thermo Fisher Scientific) according to ...
RNA was extracted from trypsynised HPAECs or lung tissue (~10 mg) using the miRCURYTM RNA Isolation Kit (Exiqon), according to the manufacturer’s instructions. To remove any residual DNA that may affect downstream applications, an On-Column DNA Removal step was performed using the RNase-...