LNA异构修饰通过糖-磷酸主链的核糖在2'-O和4'-C之间形成-O-CH2亚甲桥,从而“锁定”糖环的构象,使LNA与RNA特异结合的亲和力增强,这也是第三代化学修饰技术。最近研究显示在siRNA双链的核糖的2ʹ位置都引入LNA可延长其在血清的中的半衰期到90h,但对其基因...
Baas, "The therapeutic potential of LNA-modified siRNAs: reduction of off-target effects by chemical modification of the siRNA sequence.," Methods in Molecular Biology, vol. 487, pp. 189-203, 2009.Fluiter,K., Mook,O.R. and Baas,F. (2009) The therapeutic potential of LNA-modified siRNAs...
LNA(Locked nucleic acid)修饰属于一种构象修饰,其核心技术来自于南丹麦大学的Jesper Wengel,LNA早在1997年首先合成出来,目前应用于siRNA设计以提高对靶标的亲和力和血清稳定性等。 Jesper Wengel作为发明人拥有一系列与LNA相关的专利家族,分别保护LNA的结构通式、合成方法以及在RNAi复合物中的应用。 早期专利家族(优先权...
It incorporates important information on siRNA sequence, chemical modification, their number and respective position, structure, simplified molecular input line entry system canonical (SMILES), efficacy of modified siRNA, target gene, cell line, experimental methods, reference etc. It is developed and ...
Chemical modifications can be performed on nucleotide bases, sugar moiety, or nucleosides based on the inherent structure of ribonucleotides. Substituting uridine with 2’-O-methyl uridine (2’-OMe U) is a commonly used base modification in siRNA, which has been shown to increase siRNA stability...
The therapeutic potential of LNA-modified siRNAs: reduction of off-target effects by chemical modification of the siRNA sequence Methods Mol. Biol., 487 (2009), pp. 189-203 View in ScopusGoogle Scholar [36] S.A. Moschos, M. Frick, B. Taylor, P. Turnpenny, H. Graves, K.G. Spink, ...
LNA (Locked Nucleic Acid) are modified RNA nucleotides where the dual ribose moiety is connected via a bridge formed at the 2′ oxygen and the 4′ carbon. LNAs modification have reported to increase the base pairing affinity and reduce the ‘miRNA-like’ off-target effects [189,194]. The...
(Fig.2), can efficiently suppress immunostimulatory siRNA-driven innate immune activation, enhance activity and specificity, and reduce off-target-induced toxicity. To enhance the potency and reduce the potential toxicity of siRNA, numerous chemical modification geometries have been established and tested...
[Keywords]RNAInterference;HepatitisBVirus;siRNA;ChemicalModification;DeliveryinVivo 基金项目 作者简介 通讯作者 收稿日期 “葛根素非对映异构体单晶的HPLC-CMPA 拆分及不良反应研究”。浙江省中医药 管理局项目,编号:201OZA063。“几 种新型乌药生物碱的抗炎作用及机制研 ...
(Fig.2), can efficiently suppress immunostimulatory siRNA-driven innate immune activation, enhance activity and specificity, and reduce off-target-induced toxicity. To enhance the potency and reduce the potential toxicity of siRNA, numerous chemical modification geometries have been established and tested...