Definition Cryo‐electron microscopy (cryo‐ EM ) in combination with single‐particle reconstruction refers to the combined application of two methods used for structure determination of macromolecular assemblies: (i) cryo‐EM describes the usage of transmission electron microscopy for the visualization ...
Dr. Simon Poepsel, UC Berkeley, explains how single-particle cryo-electron microscopy enables us to gain insight into cancer development through the detailed analysis of molecular structure, covering both biology and methodology. Drug discovery webinars Learn more about drug...
Single-particle electron cryo- microscopy: towards atomic resolution. Q. Rev. Biophys. 33 (4), 307-369.Kastner, B. et al. GraFix: sample preparation for single-particle electron cryomicroscopy. Nat. Methods 5, 53-55 (2008).
Single-Particle Cryo-Electron Microscopy (Cryo-EM): Progress, Challenges, and Perspectives for Further Improvement Electron microscopy (EM) of biological macromolecules has experienced a significant increase in its capabilities with the introduction of a new generation ... D Agard,Y Cheng,RM Glaeser,...
Single-Particle Cryo-Electron Microscopy (Cryo-EM)http://linkinghub.elsevier.com/retrieve/pii/B9780128001448000021doi:10.1016/B978-0-12-800144-8.00002-1David AgardYifan ChengRobert M. GlaeserSriram SubramaniamAdvances in Imaging and Electron Physics...
The fast development of single-particle cryogenic electron microscopy (cryo-EM) has made it more feasible to obtain the 3D structure of well-behaved macromolecules with a molecular weight higher than 300 kDa at ~3 Å resolution. However, it remains a challenge to obtain the high-resolution...
Understanding the complexity of cancer with cryo-EM Download eBook Scientific webinars Hear from the scientific community using single particle cryo-EM to empower their research. View gallery Electron Microscopy Funding Support Center Access technical content, Thermo Fisher Scientific Financial Ser...
Single-particle Cryo-electron MicroscopyCatalytic ConformationRotary EnzymeV-ATPase is an ATP-driven Napump in the eubacteria(). Here, we present the first entire structure of detergent-solubilizedV-ATPase by single-particle cryo-electron microscopy (cryo-EM) using Zernike phase plate. The cryo-EM...
Single-Particle Cryo-EM at Crystallographic Resolution Cell, Volume 161, Issue 3, 23 April 2015, Pages 450-457 Yifan ChengView PDFCryo-electron microscopy (cryo-EM) of single-particle specimens is used to determine the structure of proteins and macromolecular complexes without the need for crystals...
Understanding the complexity of cancer with cryo-EM Download eBook Scientific webinars Hear from the scientific community using single particle cryo-EM to empower their research. View gallery Electron Microscopy Funding Support Center Access technical content, Thermo Fisher Scientific Financial Services, and...