作为研究工具,sdAbs在推动生物科学多个领域的发展中发挥着不可估量的作用。 这一版的《Single-Domain Antibodies: Methods and Protocols》,是2012年同名书籍的更新,分为五个部分:第一部分对sdAbs进行概述;第二部分对从几个平台分离sdAbb所...
Single-domain antibodies (sdAbs) are binders that consist of a single immunoglobulin domain. SdAbs have gained importance as therapeutics, diagnostic reagents, and research tools. Functional sdAbs are commonly produced in Escherichia coli, which is a simple and widely used host for production of ...
Anti-ICAM-1 VHH single-domain antibodies (sdAbs) are generated by immunizing a llama with recombinant ICAM-1. These antibodies are linked to an imaging moiety for in vivo or ex vivo imaging
Neutralizing antibodies provide efficient blockade for viral infection and are a promising category of biological therapies. Using SARS-CoV-2 spike RBD as a bait, we have discovered a panel of humanized single domain antibodies (sdAbs). These sdAbs revealed binding kinetics with the equilibrium ...
Tauopathies are a group of neurodegenerative diseases characterized by the presence of tau inclusions. We have developed over fifty anti-tau single-domain antibodies (sdAbs) derived from phage display libraries of a llama immunized with recombinant and p
Camelid single-domain antibodies (sdAbs, VHHs, or Nanobodies) are types of antibody fragments that are composed of the heavy-chain variable domain only. These VHHs possess unique structural and functional features, as they are small in size and exhibit thermal stability and high solubility. Comp...
Among the various antibody modalities, single-domain antibodies (sdAbs) may hold the 'key' to unlocking the access of antibody therapies to CNS diseases. The unique structural features of sdAbs make them the smallest monomeric antibody fragments suitable for molecular targeting. These features are ...
Single domain antibodies (sdAbs) are gaining a reputation as superior recognition elements as they combine the advantages of the specificity and affinity found in conventional antibodies with high stability and solubility. Melting temperatures (Tms) of sdAbs cover a wide range from below 50 to over...
single‐domain antibodydisulfide linkagethermostabilityprotein engineeringIncorporation of noncanonical disulfide linkages into single-domain antibodies (sdAbs) has been shown to enhance thermostability and other properties. Here, we evaluated the effects of introducing a novel disulfide linkage formed between ...
[15,16,17] patients. In particular, conformation-sensitive antibodies (Abs) directed against well-defined forms of Aβ42oligomers, have detected oligomers in AD brains that were absent in age-matched healthy individuals [18,19,20,21]. The aggregate distribution varies in terms of structure, ...