这一版的《Single-Domain Antibodies: Methods and Protocols》,是2012年同名书籍的更新,分为五个部分:第一部分对sdAbs进行概述;第二部分对从几个平台分离sdAbb所使用的技术进行介绍;第三部分为在生物体中制备sdAbs和相关分子的技术介绍;第...
Nevertheless, single-domain antibodies (VHHs), also known as nanobodies, present a promising alternative. VHHs possess unique characteristics over ADCs, including a smaller size, enhanced tissue penetration, and rapid clearance. Their stability, solubility, and manufacturability surpass those of ...
The crystal structure of VHH antibody is an oval shape of 4 nm×2.5 nm×3 nm in size, and its molecular weight is only 1/10 of conventional antibodies, which is 12–14 kDa, making the former the smallest intact antigen-binding fragment. Hence, the term “nanobody” was derived (Fig....
To improve efficacy, one strategy is to use smaller antibody fragments, such as single-chain variable fragments (scFv, 25 kDa) or single-domain antibodies (sdAbs or VHHs, ∼15 kDa), which have greater brain uptake albeit shorter half-life than whole antibodies [27, 28]. A few studies ...
Single-Domain Antibodies Bind HOIP RBR with High Affinities antibody screeningdAb libraries(Ignatovich et al., 2012) against biotinylated antigen captured on magnetic affinity beads. A C-terminal AviTag was introduced to the target protein, HOIP RBR (residues 697–1,072), to allowbiotinylationin ...
The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome...
Generation of human single-chain variable fragment antibodies specific to dengue virus non-structural protein 1 that interfere with the virus infectious cycle 热度: Selection and expression of recombinant single domain antibodies from a hyper-immunized library against the hapten azoxystrobin 热度: ...
(ADCs) have become one of the hotspots in the development of anticancer therapies. Although significant progress has been achieved, there remain challenges to overcome, including limited penetration into solid tumors and potential immunogenicity. Fully human single-domain antibodies (UdAbs), with their...
www.nature.com/scientificreports OPEN received: 16 May 2015 accepted: 24 July 2015 Published: 21 August 2015 Real-time analysis of epithelial- mesenchymal transition using fluorescent single-domain antibodies Julia Maier1, Bjoern Traenkle1 & Ulrich Rothbauer1,2 Vimentin has become an ...
VHH single-domain antibodies have been demonstrated to neutralize diverse bacterial toxins both and , with protein properties such as small size and high stability that make them attractive therapeutic candidates. Recently, we reported on VHHs with activity against the protective antigen component of ...