The inventive compositions can be used in methods of inducing antibody dependent cellular cytotoxicity (ADCC) in a host (e.g., to inhibit cancer or an infectious disease, such as a viral infection).DIMITROV, DIMITER S.CHEN, WEIZAOLI, WEI...
这两个NTD抗体通过抑制感染的post-attachment阶段进而阻止病毒的进入与融合。同时,COV2-2676和COV2-2489的Fab 形式失去了中和活性,这可能表明阻断是通过 Fc 段向 RBD 方向投射的空间效应间接发生的(MERS-CoV mAB 7D10 的情况一样)。后续通过丙氨酸扫描手段,确定了NTD的关键中和部位:F140S/G142D/R158S。实验团队...
To determine if the increased cross-reactivity to other α-neurotoxins translated into cross-neutralization, a single concentration antibody screen was set up using the Qube 384 system. Here, the three antibodies (368_01_C05, 2551_01_A12, and 2554_01_D11) were tested against α-cobratoxin f...
antibodies that were differentially selective for GSC. A single domain antibody VH-9.7 showed selectivity for five distinct patient-derived GSC lines and visualized orthotopic GBM xenograftsin vivoafter conjugation with a near-infrared dye. These findings demonstrate a previously unexplored high-throughput...
(e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins ...
This was necessary as there were no clear single markers that separated the EPDCs from other cells of the heart as well as epicardial cells. Using UPK3B+ cells only, we then re-calculated recall, precision and F-scores of the epicardial genes using the groups of ‘FB-like’ and ‘...
Human antibodies specific for digoxigenin, estradiol, testosterone and progesterone have been isolated from a small combinatorial IgM repertoire (4×107) of single chain antibodies (scFv). The affinities of both the anti-estradiol and anti-progesterone scFv were approximately 108 M−1. Naive IgM ge...
Potent neutralizing antibodies against SARS-CoV-2 identified by high-throughput single-cell sequencing of convalescent patients’ B cells Cell, 182 (2020), pp. 73-84.e16 View PDFView articleView in ScopusGoogle Scholar Case et al., 2020a J.B. Case, A.L. Bailey, A.S. Kim, R.E. Chen...
The single-domain Ab of camelidae is fragmentary and a therapeutic Ab. Due to the small size (~ 15 kDa) and the fast metabolism (~ 30 min) in the organisms19,20,21, appropriate modifications are essential to improve the pharmacokinetic and pharmacodynamic performances of single domain Abs...
Kong, R. et al. Epitope mapping of broadly neutralizing HIV-2 human monoclonal antibodies.J. Virol.86, 12115–12128 (2012). ArticleCASGoogle Scholar Liao, H. X. et al. High-throughput isolation of immunoglobulin genes from single human B cells and expression as monoclonal antibodies.J. Virol...