采用10X Genomics平台对免疫细胞进行scRNA-seq和TCR-seq,揭示了TCR组合对T细胞表型多样性变化的影响。 为明确TCR库的多样性是否有助于观察T细胞活化谱和整体表型多样性,研究人员以额外的3名乳腺癌患者(BC9-11)肿瘤组织样本为研究对象,利用10X Genomics平台,通过构建单细胞5’和VDJ测序文库,对流式分选(FASC)的27,000...
enabling partitioning, cell lysis, and barcoding of cellular information. These barcoded transcripts are then amplified and converted into libraries compatible with Illumina or other short-read sequencing platforms. After
Kawasaki disease (KD) is the most common cause of acquired heart disease in children in developed countries. Although functional and phenotypic changes of immune cells have been reported, a global understanding of immune responses underlying acute KD is unclear. Here, using single-cell RNA sequencin...
Using the TCR information from VDJ sequencing, we next investigated whether differential transcriptional responses to antigen stimulation could be attributed to specific clonotypes. We detected CDR3αβ sequences in 92% of analyzed T cells (69.9% fully paired CDR3αβ; 3.4% CDR3α only; 26.7% ...
Dual-Indexed Sequencing Run: Single Cell 5' v2 Dual Index libraries are dual-indexed. We do not recommend sequencing 10x Single Cell 5’ v2 Dual Index libraries with a single-index configuration. See:Can single index and dual index libraries be pooled for sequencing?
Single cell RNA sequencing provides unprecedented opportunity to simultaneously explore the transcriptomic and immune receptor diversity of T and B cells. However, there are limited tools available that simultaneously analyse large multi-omics datasets integrated with metadata such as patient and cl...
, for users new to single-cell workflows, for the development of single-cell protocols, and when working with novel cell types or complex cell systems. The system allows control over every experiment and provides the ability to troubleshoot and optimize single-cell capture without sequencing....
Second, the merge reads were assigned to VDJ germline segments and alleles from IMGT (http://www.imgt.org/) database by BLAST alignment and further re-alignment. Third, sequencing error was corrected. Then we compared clonotypes between adjacent normal tissue and tumor by calculating the top ...
utilizing single-cell RNA sequencing and B-cell receptor repertoires (BCR) analysis. We observed a significant reduction in the proportions of regulatory B cells (Bregs) and type 2 conventional dendritic cells (DCs) in patients with TAO during the active phase. Conversely, there was a significant...
Single-cell technologies, particularly single-cell RNA sequencing (scRNA-seq) methods, together with associated computational tools and the growing availability of public data resources, are transforming drug discovery and development. New opportunities are emerging in target identification owing to improved...