Kawasaki disease (KD) is the most common cause of acquired heart disease in children in developed countries. Although functional and phenotypic changes of immune cells have been reported, a global understanding of immune responses underlying acute KD is unclear. Here, using single-cell RNA sequencin...
We next assessed potential mechanisms underlying the activity of E2 vs. that of E2 + PBDE using transcriptomic analyses. RNA-sequencing was done on RNA extracted from the fourth mouse mammary gland without the lymph node (vehicle,n = 3; PBDE,n = 3; E2,n = 6; and E2...
Single Cell V(D)J Libraries (v1 and v1.1) Minimum Sequencing Depth: 5,000 read pairs/targeted cell (for more information please refer tothis guide). Single-Indexed Sequencing Run: Single Cell V(D)J v1 and v1.1 libraries are single-indexed. We do not recommend sequenc...
Single-cell RNA sequencing analysis identified 12 B-cell subtypes and characterized the transcriptomic heterogeneity of B cells in muscle-invasive bladder cancer (MIBC). The interferon-stimulated B-cell subtype (B-ISG15) and germinal center-associated B-cell subtypes (B-LMO2, B-STMN1) were signi...
respectively. 10 × Genomics platform was used for high-throughput sequencing.b, cUMAP visualization shows these cotton hypocotyl cells were grouped in to 9 clusters both in Jin668 and TM-1. Each dot indicates a single cell. Different colors indicate cell clusters.dMarker genes of each cel...
We assume raw scRNA-seq reads are mapped to genome sequences and assigned to cell barcodes and unique molecular identifiers (UMI) using pipelines that eliminate most of the UMI duplications induced by PCR and sequencing errors. We summarize all UMIs in the molecule count matrixU = [ugi]...
was counted. The libraries were prepared with the Chromium NEXT GEM Single Cell 3’ Reagent kits v3.1 according to manufacturer’s protocol. The libraries were sequenced on an Illumina Novaseq6000 platform as symmetrically paired end runs (150 bases) with 200 million raw sequencing reads per ...
Nuclei were resuspended in 0.04% BSA of PBS then stained by 4′-6′-diamidino-2-phenylindole (DAPI; Cat# D9542, Sigma-Aldrich) for counting and diluted in an appropriate concentration. Single cell sorting, reverse transcription, amplification and sequencing The cryopreserved single-cell suspension ...
there may have been a survival bias among microglial subpopulations that are successfully profiled; this can be investigated further in the future using complementary approaches such as single nucleus RNA-sequencing. Our immunofluorescence studies begin to address this point and suggest that the frequenci...
bulk analyses of pre-selected cell types provide a view of the averaged global gene expression patterns from the cell population within the tissue type but do not differentiate the cell types in the sample. Single-cell RNA-sequencing (scRNA-seq) has revolutionized our ability to comprehensively an...