Single cell RNA-SeqPeripheral blood mononuclear cellsCase reportHepatitis B Virus-related acute-on-chronic liver failure (HBV-ACLF) is a severe complication with high fatality rates. However, the underlying mec
In parallel, to better determine the contribution of type I IFN secretion to the transcriptomic profile of SAVI patients, we performed single cell RNA sequencing (scRNA-seq) on PBMCs from three CTRLs, stimulated with human recombinant IFN-β at different timepoints (IFN-β dataset) (Figure 1...
Here, a single-cell RNA sequencing analysis of PBMCs from 4 AD patients (2 in the early stage, 2 in the late stage) and 2 normal controls was performed to explore the differential cell subpopulations in PBMCs of AD patients. A significant decrease in B cells was detected in the blood ...
Kawasaki disease (KD) is the most common cause of acquired heart disease in children in developed countries. Although functional and phenotypic changes of immune cells have been reported, a global understanding of immune responses underlying acute KD is unclear. Here, using single-cell RNA sequencin...
Current single-cell RNA-sequencing approaches have limitations that stem from the microfluidic devices or fluid handling steps required for sample processing. We develop a method that does not require specialized microfluidic devices, expertise or hardwa
Fig. 1: Single-cell RNA sequencing allows detection of transcriptional shifts induced by antigen-specific stimulation in TCR-barcoded clonotypes. a Experimental setup; cells from tracheal aspirates and peripheral blood T cells (flow cytometry-sorted for CD4 or CD8 positivity) of intensive care unit...
Advances of individual-cell RNA sequencing in carcinoma Immune cells Non-immune cells Combination of scRNA-seq and other methods Use of individual-cell RNA sequencing inside different fields Conclusions and perspective Author contributions Declaration of interests ReferencesShow full outline Cited by (59)...
Single-cell RNA sequencing Libraries for scRNA-seq were prepared using the Chromium Single Cell 5′ Library & Gel Bead Kit (PN-1000014, 10X Genomics), Chromium Single Cell A Chip kit (PN-1000009, 10X Genomics), and Chromium i7 Multiplex Kit (PN-120262, 10X Genomics). Samples were loaded ...
Liu et al. Cell Discovery (2022)8:11 https://doi.org/10.1038/s41421-021-00362-2 Cell Discovery www.nature.com/celldisc ARTICLE Open Access Single-cell RNA sequencing identifies an Il1rn+/ Trem1+ macrophage subpopulation as a cellular target for mitigating the progression of thoracic aortic ...
Moreover, using independent single-cell RNA sequencing (scRNA-seq) and single-cell BCR sequencing (scBCR-seq) datasets from primary and secondary natural DENV-infected patients [17], we demonstrated a positive relationship between tissue-homing gene expression and ASC clonal expansion, with the ...