Lipson D, Bonnar JL, Jost M, Norman TM, Weissman JS. Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq. Cell. 2022 Jun 2:S0092-8674(22)00597-9. doi: 10.1016/j.cell.2022.05.
we downsampled to specific numbers of reads per cell, and numbers of cells per gRNA. 分析的结果表明the same performance in differential gene expression testing is achieved in TAP-seqat a 19- to 49-fold lowerread depth than that of Perturb-seq。总...
流式细胞仪的cell sorting是很多细胞和分子生物实验的基础,比如单细胞测序、细胞周期等。 Flow cytometry and cell sorting are two distinct yet complementary techniques. Both rely on antibodies to detect specific cells within a heterogeneous population, but while flow cytometry measures the proportion of each...
由于盒大小的限制,CROP-seq被认为与多种sgRNA的递送不兼容。 第一批应用:Perturb-seq: Dissecting molecular circuits with scalable single cell RNA profiling of pooled genetic screens - 2017 - Cell 第一代其他: Dissecting Immune Circuits by Linking CRISPR-Pooled Screens with Single-Cell RNA-Seq - Scie...
2016 年 12 月,基于 10x Genomics Chromium single cell 平台,第一篇文章发表于 Cell,文章将单细胞 RNA 测序与基于 CRISPR 扰动(称为 Perturb-seq)技术相结合,大规模的分析复杂表型,研究基因修饰对细胞状态的影响。 同期《Cell》,在 Dixit A 等人发表的文章后,另一篇基于 10x 平台,通过 CRISPR 系统研究未折叠...
However, experimental and statistical limitations constrain sensitivity and throughput of genetic screening with single-cell transcriptomics readout. To overcome these limitations, we introduce targeted Perturb-seq (TAP-seq), a sensitive, inexpensive and platform-independent method focusing single-cell RNA-...
In two papers published today in Cell, two teams working collaboratively from UCSF and the Broad Institute describe Perturb-seq, a novel approach to high-throughput functional genomics enabled by the combination of single cell RNA-seq with CRISPR perturbations. Both teams took advantage of the mas...
Massively parallel phenotyping of coding variants in cancer with Perturb-seq. Nat. Biotechnol. 40, 896–905 (2022). High-throughput analysis of oncogene and tumour suppressor variant phenotypes at single-cell level. Article CAS PubMed Google Scholar Chaligne, R. et al. Epigenetic encoding, ...
However, experimental and statistical limitations constrain sensitivity and throughput of genetic screening with single-cell transcriptomics readout. To overcome these limitations, we introduce targeted Perturb-seq (TAP-seq), a sensitive, inexpensive and platform-independent method focusing single-cell RNA-...
This work was described in a pair of papers that appeared December 15 in the journal Cell. In the Broad-led study (“Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens”), researchers used CRISPR/Cas9 nucleases to cut DNA and inactivate...