Here we describe single-cell corrected long-read sequencing (scCOLOR-seq), which enables error correction of barcode and unique molecular identifier oligonucleotide sequences and permits standalone cDNA nanopore sequencing of single cells. Barcodes and u
Combining single-cell RNA sequencing with Nanopore long-read sequencing enables isoform-level analysis in single cells. However, due to the higher error rate in Nanopore reads, the demultiplexing of cell barcodes and Unique molecular Identifiers (UMIs) can be challenging. This tool enables the accur...
Sequencing of nucleic acids with nanopores has emerged as a powerful tool offering rapid readout, high accuracy, low cost and portability. This label-free method for sequencing at the single-molecule level is an achievement on its own. However, nanopores
scNanoGPS is a computational toolkit for analyzing high throughput single cell nanopore sequencing data to detect Genotypes and Phenotype Simultaneously from same cells. scNanoGPS includes 5 major steps: 1) NanoQC to perform quality control of the raw seqeucning data; 2) Scanner to scan and fil...
Single-cell sequencingAlternative splicingTranscript isoformTranscript isoforms are mRNAs that arise from alternative splicing events. During RNA processing, different combinations of a gene's exons lead to a diverse set of isoforms. Polymorphisms or mutations at splice junctions can generate alternative ...
2020 年4 月,西班牙科学家在 Nature 杂志上发表了一篇论文Benchmarking single-cell RNA-sequencing protocols for cell atlas projects论述了不同的单细胞测序技术的差异,文章比较了13种常用scRNA-seq和单核RNA-seq实验方案。比较分析显示实验方案的性能存在明显差异。这些实验方案在文库的复杂性及其检测细胞类型标记的...
3、Single-Cell Genotyping by Short-Read and Nanopore Sequencing (短读和纳米孔测序的单细胞基因分型) 之前的肿瘤的单细胞数据已经检测了基因的突变(转录组全长)CNVs来识别恶性细胞,而3‘端高通常的测序方法,限制了突变的检测,而且,AML缺少CNVs信息,因此,我们采用了Seq-Well来扩增和测序包含AML突变的转录本部分...
This guide introduces the single-cell RNA nanopore sequencing workflow, for ultra-rich data without compromise.
Mol. Cell. Proteom. 21, 100230 (2022). Article Google Scholar Boparai, J. K. & Sharma, P. K. Mini review on antimicrobial peptides, sources, mechanism and recent applications. Protein Pept. Lett. 27, 4–16 (2020). Article Google Scholar Koch, C. et al. Nanopore sequencing of ...
information to single-cell spatial omics such as isoform usage and mutation status with the use of RaCH-seq. By integrating long nanopore sequencing reads to already established high-throughput single-cell technologies, we are another step closer to resolving cancer complexity and improving patie...