2000. Gene-expression analysis at the single-cell level. Trends Pharmacol. Sci. 21:65- 70.DIXON, A. K., RICHARDSON, P. J., PINNOCK, R. D. & LEE, K. (2000). Gene-expression analysis at the single-cell level. Trends in Pharmacological Sciences 21, 65-70....
Most current methods of measuring gene expression rely on averaging many cellular responses or artificial amplification steps to reach a detectable threshold of signal. In contradistinction, in situ assays circumvent these procedures to yield direct single cell expression information. Fluorescence in situ h...
Recent advances in single-cell technologies have enabled high-throughput molecular profiling of cells across modalities and locations. Single-cell transcriptomics data can now be complemented by chromatin accessibility, surface protein expression, adapti
在一些研究项目中我们还可以进行细胞亚群差异基因的探索,点击Differential expression按钮交互: 图源:https://singlecell.zendesk.com/hc/en-us/articles/6059411840027-Exploratory-differential-gene-expression-analysis 选择细胞亚群如T细胞,将T细胞与其他细胞亚群进行对比分析,显示前15个差异表达最多的基因: 图源:https://...
Genome-wide analysis of gene expression RNA sequencing Statistical methods Transcriptomics This article is cited by The effect of data transformation on low-dimensional integration of single-cell RNA-seq Youngjun Park Anne-Christin Hauschild BMC Bioinformatics(2024) ...
欧易生物正式推出 10× Genomics Single Cell Gene Expression Flex单细胞转录组测序技术(以下简称FLEX),采用4%的多聚甲醛(PFA)对细胞进行固定,一步锁定RNA表达状态, 解决了样本储存、多样本批次处理的问题,而且还配备更加实惠的混样测序整体解决方案,并同时解锁FFPE样本的单细胞转录组测序功能!
(A) Scheme used for single-cell gene expression analysis with Fluidigm. (B) Representative images of NGFP2 MEFs without dox and at days 2, 4, and 6 on dox. (C) Scheme of NGFP2/tdTomato secondary system used to measure single-cell gene expression of clonal dox-dependent (GFP−, GFP...
Analysis of 11 genes in serum-stimulated cultured cells revealed unique patterns of gene expression within individual cells. Using the nucleus as the substrate for parallel gene analysis, we provide a platform for the fusion of genomics and cell biology: "cellular genomics." 展开 关键词:...
只需要好好理解Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Kits User Guide,17-20页的内容即可。 2023年10月25日 Change location of 'fragments' in multiome Seurat file更新fragment文件位置 Reset the current fragments object in the Seurat ...
For a single-modal module, only a single modality like gene expression in the cell can be obtained for analysis. Imputation, cell type annotation, and clustering tasks are supported under this module. For the multimodal module, multiple modalities for the cell can be accessed. For example, ...