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Single-Cell Expression Profiling at Defined Time Points To measure gene expression in single cells at defined time points during the reprogramming process, we combined two complimentary tools: (1) 96.96 Dynamic Array chips (Fluidigm), which allows quantitative analysis of 48 genes in duplicate in 96...
在一些研究项目中我们还可以进行细胞亚群差异基因的探索,点击Differential expression按钮交互: 图源:https://singlecell.zendesk.com/hc/en-us/articles/6059411840027-Exploratory-differential-gene-expression-analysis 选择细胞亚群如T细胞,将T细胞与其他细...
Integration of single-cell RNA sequencing data between different samples has been a major challenge for analyzing cell populations. However, strategies to integrate differential expression analysis of single-cell data remain underinvestigated. Here, we benchmark 46 workflows for differential expression analy...
在一些研究项目中我们还可以进行细胞亚群差异基因的探索,点击Differential expression按钮交互: 图源:https://singlecell.zendesk.com/hc/en-us/articles/6059411840027-Exploratory-differential-gene-expression-analysis 选择细胞亚群如T细胞,将T细胞与其他细胞亚群进行对比分析,显示前15个差异表达最多的基因: ...
Single Cell Gene Expression Profiling: Multiplexed Expression Fluorescence in situ Hybridization (FISH) Application to the Analysis of Cultured Cells Jeffrey M Levsky , Steven A Braut and Robert H Singer I.Introduction Most current methods of measuring gene expression rely on averaging many cellular re...
4.3Single-cell analysis While thin sectioning andLCMallow for the isolation of smaller sections from a larger biofilm sample, cells from different subpopulations may still end up together in the same section.Single-cell analysissidesteps this problem by examining gene expression in only one cell. Si...
只需要好好理解Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Kits User Guide,17-20页的内容即可。 2023年10月25日 Change location of 'fragments' in multiome Seurat file更新fragment文件位置 Reset the current fragments object in the Seurat ...
Branch expression analysis modeling (BEAM) was used to analyze the pseudo-time-dependent or branch-dependent genes. The genes that were significantly branch-dependent were visualized by the “plot_genes_branched_heatmap” function. RNA velocity analysis...
Since the amount of RNA from a single cell is very limited, scRNA-seq depends largely on amplification. Such large amounts of RNA amplification causes random dropout events, thus inducing a large number of zero counts in the expression matrix. Therefore, the analysis of scRNA-seq data needs ...