确定一下 cell 名的组成,构建好 metadata(行名) 和 count(列名) 矩阵能够对应 过滤掉外源 RNA (ERCC) 合并成为 seurat 对象 计算线粒体(MT),核糖体的 RNA(^RP[SL]) 比例并筛选数据 我们先处理第一个表达矩阵 # 这个是治疗前的第一个表达矩阵 # 导入数据 raw.data = read.csv('csv_files/S01_datafin...
Single Cell Expression Atlas: https://www.ebi.ac.uk/gxa/sc/home SignatureDB: https://lymphochip.nih.gov/signaturedb/ KIT(肾脏单细胞数据库): http://humphreyslab.com/SingleCell/ 血管细胞数据库: http://betsholtzlab.org/VascularSingleCells/database.html 脑细胞数据库: https://web.stanford.e...
Chromium Single Cell Gene Expression provides single cell transcriptome 3' gene expression alongside the detection of surface protein expression or CRISPR edits in tens of thousands of cells. Scale experiments up or down with low-throughput and high-thro
singlecell_endodiff_paperPublic Single-cell RNA-sequencing of differentiating iPS cells reveals dynamic genetic effects on gene expression limix_qtlPublic cellsnp-litePublic Efficient genotyping bi-allelic SNPs on single cells vireoPublic Demultiplexing pooled scRNA-seq data with or without genotype refere...
Single Cell Gene Expression Profiling: Multiplexed Expression Fluorescence in situ Hybridization (FISH) Application to the Analysis of Cult Single Cell Gene Expression Profiling: Multiplexed Expression Fluorescence in situ Hybridization (FISH) Application to the Analysis of Cultured Cells ...
Recent advances in single-cell RNA sequencing have shown heterogeneous cell types and gene expression states in the non-cancerous cells in tumors. The integration of multiple scRNA-seq datasets across tumors can indicate common cell types and states in t
We then computed Spearman’s correlation coefficient between each cell type-specific transcriptome and the average expression profile of each transformed subpopulation across all eight tumors in our data set. All cell types with a below-median standard deviation were then removed to enrich for cell ...
Single-cell RNA sequencing (scRNA-seq) can be used to characterize variation in gene expression levels at high resolution. However, the sources of experimental noise in scRNA-seq are not yet well understood. We investigated the technical variation associ
In theGene Expressionview, running the human-mouse mixture experiment results in the followingGEM Partitionsplot and metrics: Each point represents a barcode. The gray points are inferred to be associated with GEM partitions that contain more than one cell because they contain a large number of mo...
欧易生物正式推出 10× Genomics Single Cell Gene Expression Flex单细胞转录组测序技术(以下简称FLEX),采用4%的多聚甲醛(PFA)对细胞进行固定,一步锁定RNA表达状态, 解决了样本储存、多样本批次处理的问题,而且还配备更加实惠的混样测序整体解决方案,并同时解锁FFPE样本的单细胞转录组测序功能!