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Algorithms for whole genome shotgun sequencing A monumental achievement in the history of science, the sequencing of the entire human genome, will soon be reached. The Human Genome Project (HGP) has been working toward this goal since 1990 using a two-tiered strategy. Recently it was... E ...
genome by 2005, began in 1990. The cost was initially enormous. The entire project was projected to cost $1.5 billion or approximately $10 per sequenced base. Craig Venter of Celera Genomics completed the sequencing of the human genome several years ahead of the official Human Genome Project ...
Project annotation consortium: Human cancer genome project sequencing consortium. The generation and utilization of a cancer-oriented representation of the... Open reading frame expressed sequences tags (ORESTES) differ from conventional ESTs by providing sequence data from the central protein coding porti...
Shotgun sequencinghas been introduced recently to complement the ongoing clone-by clone sequencing effort. Two types of genomic library have been constructed for this project: a whole-genome shotgun library subtracted with highlyrepetitive sequences, and a shotgun library derived from a mixture of ...
microbial community largely unknown. Here, we are particularly interested in exploring the taxonomic composition and metabolic potential of microorganisms in soil from Manao-Pee cave, a subterranean limestone cave in the western part of Thailand, by using high-throughput shotgun metagenomic sequencing. ...
DNA sequencing, first-draft sequence, contig, completion, WGS, scaffolds in genome sequencing, human genome, genome projects; Bankier AT 2001 Methods Mol Biol 167:89; whole genome shotgun (WGS) products: http://www.ncbi.nlm.nih.gov/projects/WGS/WGSprojectlist.cgi... This is a preview of...
A complete bacterial genome assembled de novo using only nanopore sequencing data. Nat. Methods 12, 733–735 (2015). Article CAS PubMed Google Scholar Kuleshov, V. et al. Synthetic long-read sequencing reveals intraspecies diversity in the human microbiome. Nat. Biotechnol. 34, 64–69 (...
Human papillomavirus community in healthy persons, defined by metagenomics analysis of human microbiome project shotgun sequencing data sets. Ma Y; Madupu R; Karaoz U;.Human papillomavirus community in healthy persons, defined by metagenomics analysis of human microbiome project shotgun ... Y Ma,R...
To reduce sequencing reads mapping to eukaryotic DNA downstream, DNA from each swab was treated with the Microbiome Enrichment kit (NEB) and purified with AMPure magnetic beads (Beckman Coulter). A negative sterile water control was included during the enrichment and carried forward through ...