1. Introduction One of the most important attributes of the polymerase chain reaction (PCR) is its exquisite sensitivity. However, this high sensitivity has also given PCR its main weakness, namely, its tendency to produce false-positive results due to exogenous contamination (1,2). Contamination ...
An issue in molecular genetics laboratories is mentioned to be the minimization of risk of contamination by amplicons from polymerase chain reaction (PCR). The traditional applications of radioactivity can be performed by non-radioactive methods and long range PCR can replace some southern blot ...
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Factors Influencing PCR Success Contamination Cycle parameters Primers Avoiding Contamination Sample preparation, reaction mixture assemblage should be performed in separate areas. A Laminar Flow Cabinet with a UV lamp is recommended for preparing the reaction mixture. New gloves should be used for DNA/R...
A nested-polymerase chain reaction (PCR) has been set up to be performed in a single closed tube for the detection of Pseudomonas savastanoi pv. savastanoi... E Bertolini,R Penyalver,A Garc??A,... - 《Journal of Microbiological Methods》 被引量: 111发表: 2003年 Expression of the α6...
Polymerase chain reaction (PCR) in ocular and ganglionar toxoplasmosis and the effect of therapeutics for prevention of ocular involvement in South updates... Polymerase chain reaction (PCR) in ocular and ganglionar toxoplasmosis and the effect of therapeutics for prevention of ocular involvement in ...
In the early phase of the COVID-19 outbreak, when reverse transcription polymerase chain reaction (RT-PCR) tests had limited availability and the number of infected people was higher, initial efforts focused on the use of imaging examinations at the forefront of diagnosis. Since the lungs are ...
Setting up a PCR laboratory. INTRODUCTIONDevelopment of the polymerase chain reaction () as a basic component of the molecular biology laboratory has occurred very rapidly from its inc... CW Dieffenbach,GS Dveksler - 《Pcr Methods & Applications》 被引量: 171发表: 2003年 ...
ait is usually necessary to isolate physically the PCR reaction and detection systems from the other steps of the procedure (and, indeed from the main laboratory) 完全隔绝PCR反应和检测系统从做法的其他步和的确从 (主要实验室通常是必要的)[translate] ...
Samples with no signal detected during the real time RT-PCR are shown at Ct 45 in the graphs. Full size image The results of the comprehensive study which examined 10 spikes each at both 15.6 and 31.25 copies/reaction performed in duplicate for a total of 20 samples per spike amount are ...