In case of growth of AFB on solid or in fluid media PCRs and subsequent sequencing of the targets 16 S ribosomal DNA24 and myco- bacterial gyrase B subunit DNA, gyrB25, were performed to differentiate between the mycobacteria species of the MTB complex. Non-tuberculous mycobacteria were ...
To explore the role of riceSQD2.2encoding a putative SQDG synthase, a full-length cDNA of riceSQD2.2was cloned and overexpressed in rice under the control of the maizeUbiquitinpromoter. A total of 41 transgenic lines were obtained and were confirmed by PCR. The sequencing result of PCR product...
All animals were fed twice a week with newly hatched brine shrimp, and seawater was changed twice per week. To identify the clade present, DNA was extracted from symbiotic A. diaphana using the DNeasy kit (Qiagene, Germany) and analysed by RT-PCR, using a set of four clade-specific ...
cDNA was made by reversing mRNA (Qiagen), and qRT-PCR was performed in a thermocycler with SYBR Green PCR Master Mix (Qiagen). The comparative cycle threshold (Ct) (2−ΔΔCT) was used to calculate the relative expression of TGF-β mRNA and COL1A1 mRNA. Table 2 summarises the ...
In luminal B HER2-negative patients, relatively low baseline serum levels of miR-375-3p were found to be associated with pathological complete response (pCR) (p = 0.043) and “comprehensive response”, defined as partial or complete response in clinical evaluation and loss > 30% or no ...