How to Choose the Right PCR ReagentsTanuja Koppal, Ph.D
After adding the nucleic acid to be detected, the reaction system performs a fluorescent PCR amplification reaction. The time is slightly different depending on the PCR reagents and instruments used. Generally,it takes 90-120 mi...
Clean-up of PCR reaction Once the template DNA has been amplified by PCR, clean-up is required to remove unincorporated primers; any leftover primers can interfere with the strand-specific cycle sequencing reactions that will follow. There are several m...
Polymerase chain reaction (PCR) is a biochemical process that uses repeated cycles of heating and cooling to amplify a genetic region of interest, called the amplicon. In each cycle the double stranded DNA is first denatured and then cooled, allowing short oligonucleotide primers to anneal to the...
Polymerase Chain Reaction is the in-vitro method of making multiple copies of a DNA sequence. The three main phases of PCR are Denaturation, Annealing, and Elongation.Answer and Explanation: During PCR, the function of DNA polymerase is the add the dNTPs (deoxyribonucleotide triphosphates) to ...
When real-time polymerase chain reaction came out during the 90s the words kinetic PCR analysis or real-time PCR were most commonly used [1][2][3]. Also, real-time quantitative RT-PCR was used to indicate RT-qPCR [4]. When real-time PCR became more common in the 2000s it was more...
Heterodimeric capping protein (CP/CapZ) is an essential factor for the assembly of branched actin networks, which push against cellular membranes to drive a large variety of cellular processes. Aside from terminating filament growth, CP potentiates the n
These DNA substrates were consequently introduced into a human cell line (HCT 116), and joining was characterized both in terms of efficiency, by quantitative PCR (qPCR) of head-to-tail products, as well as product structure, by sequencing (Fig. 2a and Supplementary Fig. 2). We used ...
Each restriction enzyme has optimal reaction (assay) conditions and different conditions for long term storage. The recommended assay and storage conditions are both determined by the manufacturer to provide the user with the highest activity, best fidelity and greatest stability for each enzyme. Factor...
Tip:After addition of Reagent 2, the samples must be used immediately or frozen at -20 ºC. To reduce sample dilution, samples can be added to Reagent 2 at a ratio of 1:3, 1:2 or 1:1. Remarks The PCR reaction can be run immediately or go directly to other downstream technique...