In vitro, podocytes were stimulated with PEDF under normal or high-glucose conditions; permeability was measured by the transwell assay with fluorescein isothiocyanate (FITC)-dextran; and F-actin cytoskeleton was analyzed by phalloidin staining. Apoptosis was assessed by flow cytometry. RhoA activity ...
levels of KRAS and FcRn to albumin-conjugated doxorubicin (DOX-ALB) and free DOX was investigated. Suitable chemical and physical stability of DOX-ALB was yielded with conjugation of Cys 34 to DOX using uncleavable linkers (Mal-PEG-NHS). Enhanced micropinocytosis by evaluating F...
High levels of immune complexes (IC) wer... B Galvo-Castro,JAS Ferreira,KF Marzochi,... - 《Clinical & Experimental Immunology》 被引量: 304发表: 1984年 Intestinal Transmission of Macromolecules (BSA and FITC-Dextran) in the Neonatal Pig: Enhancing Effect of Colostrum, Proteins and ...
Stimulation of dHL-60 cells with PMA significantly increased NETosis and the supernatant MPO-DNA and nucleosome levels compared with the control (p < 0.001) (Fig. 4A). Next, we treated dHL-60 cells with serum from HD patients (n = 40) and HV (n = 20) and evaluated NET...
Significant levels of SAA1/SAA2 RNA and SAA protein were detected by in situ hybridization and immunohistochemistry in mouse colonic epithelium. SAA3 expression was weaker, but similarly distributed. SAA1/2 RNA was present in the ileum and colon of conventional mice and in the colon of germfree...
levels of C5a (Apcs−/−, 22.7 ± 1.7 vs. wt, 31.5 ± 1.8 ng/ml;P = 0.004; 16 h) (Supplementary Fig.4b). Decreased C3d in lung lysates was also observed (P = 0.03) (Supplementary Fig.4c). Murine SAP pre-opsonisation restored the levels of MPO ...
(FITC)-labeled dextran.Transmission electron microscopy was used to observe the ultrastructure of tight junctions(TJs).Immunofluorescence of zonula occludens-1(ZO-1),claudin-1 and nuclear transcription factor-kappa p65(NF-κBp65)were measured to determine the protein distribution.The mRNA expression ...
2D). We showed that the significant increase in plasma C3a levels (Fig. 4A) is due to inhibition of FH-mediated complement regulation of HDL particles by FH5–7. In the current manuscript we show to our knowledge for the first time, protection of HDL particles in plasma against the ...
(MPS) was taking as the positive control. Levels in the NEP1-40-MP-NP group were significantly lower than in the other treatment groups. These results indicate that MPS and MP encapsulated in NPs can alleviate free radical damage in rats after SCI, with NEP1-40-MP-NPs showing the ...
In vitro, podocytes were stimulated with PEDF under normal or high-glucose conditions; permeability was measured by the transwell assay with fluorescein isothiocyanate (FITC)-dextran; and F-actin cytoskeleton was analyzed by phalloidin staining. Apoptosis was assessed by flow cytometry. RhoA activity ...