Oral administration of fluorescein isothiocyanate dextran (FITC-d) has been used as an indicator for intestinal permeability in poultry research for several years. Under healthy conditions, tight junctions in the intestinal wall will not allow the 4-6kDa FITC-d to enter the bloodstream. Detection ...
In vitro, NETs increase the flux of albumin and 10 kDa dextran across EC monolayers. Neutralizing NET components by DNase 1, or inhibition of NET formation by a PAD2/4 inhibitor or PAD4 gene deletion, reduces lung vascular permeability in murine models of transfusion-related acute lung injury...
In these studies, NEP1-40 was labeled with FITC that could be detected in the green channel, while MP-NPs were labeled with Alexa Fluor® 555 that could be detected in the green channel. Co-localized red and green puncta were observed in cells treated with NEP1-40-MP-NPs, suggesting ...
(FITC)-labeled dextran.Transmission electron microscopy was used to observe the ultrastructure of tight junctions(TJs).Immunofluorescence of zonula occludens-1(ZO-1),claudin-1 and nuclear transcription factor-kappa p65(NF-κBp65)were measured to determine the protein distribution.The mRNA expression ...
Enhanced micropinocytosis by evaluating FITC-Dextran as a macropinocytosis marker and elevated albumin uptake and degradation level was demonstrated in KRASG12V-overexpressing BxPC-3 cells. Further study in human umbilical vein endothelial cells (HUVECs) verified the protection role of FcR...
Dextran sodium sulfate (DSS) colitis was induced in SAA 1/2 double knockout (DKO) mice and in wildtype controls. Anti-bacterial effects of SAA1/2 were tested in intestinal epithelial cell lines transduced with adenoviral vectors encoding the CE/J SAA isoform or control vectors prior to ...
Epithelial barrier function was assessed by transepithelial electrical resistance(TER) and permeability of fluorescein isothiocyanate(FITC)-labeled dextran. Transmission electron microscopy was used to observe the ultrastructure of tight junctions(TJs). Immunofluorescence of zonula occludens-1(ZO-1), claudin...
(FITC)-labeled AF conidia;n = 3 mice). Left, merged image of a lung stained for SAP (red), C3 (blue) and neutrophils (Ly6G+cells; cyan). Conidia are evident from the detection of FITC (green) signal. Dashed white arrowheads indicate regions of SAP localization on AF conidia. A...
Intestinal Transmission of Macromolecules (BSA and FITC-Dextran) in the Neonatal Pig: Enhancing Effect of Colostrum, Proteins and Proteinase Inhibitors The effects of colostrum and constituents/factors in colostrum which may influence intestinal macromolecular transmission in the newborn preclosure pig wer...
dextran conjugate, not by PTX, because no acute death was found for the commercial PTX injec- tion. However, in our previous study, we have used BSA–dextran conjugate and doxorubicin hydrochlo- ride to produce doxorubicin-loaded nanoparticles ...