4. Calculate FITC-dextran concentration based on fluorescence intensity.5. Calculate permeability. In Vivo: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).For intestinal barrier function assay1. Fast ...
The FITC-dextran concentration of the mother liquor was measured over a period of hours and days. In this study, one- and two-compartment models were developed, based on a species mass balance, to predict the concentration of dextran release from the microcapsules over time. The two-...
To better quantitate the effect of BK on macromolecular transport, the superfusate output was collected at 5-min intervals and analyzed by fluorometry to determine FITC-Dextran 150 concentration. After BK, fluorochrome concentration in the superfusate rose, peaked by 15–20 min, and returned to...
The FD-4 method utilizes that simple sugars passively move from the gut to the circulation without being metabolized or degraded. The amount passing over the intestinal barrier depends on the barrier’s concentration gradient, surface area, mucosal blood flow, transit time, and intestinal permeability...
It was shown that the presence of SDS in the medium, at a concentration of 3000 μg/ml, leads to the destruction of polyelectrolyte microcapsules and the release of the substance from them (54% of the amount of the encapsulated substance), while the main part of the FITC-dextran released...
BK was added to the buffer solution at the concentration of 2 micrograms/ml five minutes after a control period of 60 minutes. Ischemia was performed during 60 min by a clamp positioned on the main artery of the cremaster muscle. Animals treated with Daflon 500 mg (100 mg/kg) 18 and 2...
2. Suspend the cells in 100 μL of medium, and mix in Q-prep tubes with 10 μL of propidium iodide (PI), 10 μL of FITC-Dextran (MW 4000) (the final concentration of PI and FITC-Dextran (MW 4000) is 7.5 μM and 1.13 μM, respectively).3. Incubate cells for 25 min at ...
Internal porosity of microspheres decreased considerably with increasing polymer concentration. Increase in homogenization speed during the primary emulsion preparation led to decreased internal porosity. Burst release decreased with increasing drug loading but increased with drug molecular weight. Drug ...
4. Calculate FITC-dextran concentration based on fluorescence intensity.5. Calculate permeability. In Vivo: Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).For intestinal barrier function assay1. Fast ...
The intestinal transmission was assessed as the serum concentration of the markers after 4 or 6 hours, when the uptake of FITC-dextran into the enterocytes was also examined by fluorescence microscopy.ksrömWsrömmoKarssonNaturePediatric Research...