1. Add FITC-dextran (0.1 mg/mL) to the basal media in the transwell chamber.2. Collect media from the transwell insert after 15 min.3. Measure the fluorescence signal (Ex=485 nm, Em=538 nm).4. Calculate FITC-dextran concentration based on fluorescence intensity.5. Calculate permeability....
Some nanoliters of FITC-dextran mean mol wt ( M w) 20,000, 3000, or sodium fluorescein ( M w 376) were injected into an arteriole of the exposed cheek pouch via micropipet. The extravasation of fluorochromes was measured by a photodensitometric method including two sets of calibration ...
Fig. 2: FITC dextran 4kDA intestinal permeability test. AC57BL/6 J mice on a low-fat diet (LFD) were weight stratified in two groups, and FITC dextran 4 kDa (FD-4) were dosed PO. in a low dose or high dose, respectively (n = 10/grp). 2 samples were excluded due to...
To better quantitate the effect of BK on macromolecular transport, the superfusate output was collected at 5-min intervals and analyzed by fluorometry to determine FITC-Dextran 150 concentration. After BK, fluorochrome concentration in the superfusate rose, peaked by 15–20 min, and returned to...
It was shown that the presence of SDS in the medium, at a concentration of 3000 μg/ml, leads to the destruction of polyelectrolyte microcapsules and the release of the substance from them (54% of the amount of the encapsulated substance), while the main part of the FITC-dextran released...
FITC-Dextran 150 (MW 150,000) was injected i.v. as a macromolecular tracer. BK was added to the buffer solution at the concentration of 2 micrograms/ml five minutes after a control period of 60 minutes. Ischemia was performed during 60 min by a clamp positioned on the main artery of ...
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Free Radical Biology and Medicine Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis Pubmed:24992835 Respiratory Virology and Immunogenicity Advances in Experimental Medicine and Biology Cytokines and Toll-Like Receptors in the...
7. Add xylene cyanolto a final concentration of 0.1%(m/v) and glycerol to 5%(m/v). xylene cyanol 0.0042g,显色作用,使过柱时蛋白质与FITC易于分辨; (本实验未加,最下层为蛋白质) glycerol 甘油 m2= (2.0+0.15) mL×5%=0.1075g (2mL离心管称取) ...
7. Add xylene cyanolto a final concentration of 0.1%(m/v) and glycerol to 5%(m/v). xylene cyanol 0.0042g,显色作用,使过柱时蛋白质与FITC易于分辨; (本实验未加,最下层为蛋白质) glycerol甘油m2= (2.0+0.15) mL×5%=0.1075g (2mL离心管称取) ...