FITC-dleaky gutpigmentpoultryserumOral administration of fluorescein isothiocyanate dextran (FITC-d) has been used as an indicator for intestinal permeability in poultry research for several years. Under healthy conditions, tight junctions in the intestinal wall will not allow the 4-6kDa FITC-d to ...
Colitis was induced in 10 weeks old male DKO mice or controls, by adding 3% dextran-sodium sulfate (DSS; molecular weight 40,000 Da; ICN Biomedicals, Aurora, Ohio, USA) to the drinking water, for 7 days. Controls received untreated water. The mice were examined on day 9 for weight loss...
Uptake of free NEP1-40and NEP1-40-MP-NPs by Nogo-positive RSC-96 cells was analyzed. As a negative control, we performed the same experiments in Nogo-negative human umbilical vein endothelial cells (HUVECs). In these studies, NEP1-40was labeled with FITC that could be detected in the ...
FITC-dextranIntestinal permeabilityMacrophage migration inhibitory factorTransmission electron microscopyMacrophage migration inhibitory factor (MIF) is a molecule with multiple functions: from enforcing the immune system to fight bacterial infection to the regulation of insulin activity. Also, MIF is......
Given a similar size, cationic compounds are more easily filtered than neutral or anionic compounds due to the strong net negative charge at the site of the glomeruli, as demonstrated by dextran (radius of ±3.6 nm) and horse radish peroxidase (radius of ±3 nm) ranging in pI below 4 up...