Wangikar PP, Carmichael D, Clark DS, Dordick JS. 1996. Active-site titration of serine proteases in organic solvents. Biotechnol Bioeng 50:329-335.Wangikar PP,Carmichael DS,Douglas C,Dordick JS.Active- sitetitration of serine protease in organic solvents. Biotechnology and Bioengineering ...
of thisenzymehas been later cloned by RT-PCR[33]. The protease activity of developing and dry seeds was sensitive to PMSF and EDTA, as well as to synthetic poly-L-Glu—just like the purified protease C1. Those authors suggested that it was synthesized in a form that is active in vitro...
Figure 3: Protease mechanisms Polypeptides can be cleaved either chemically or enzymatically. Enzymes that catalyse the hydrolytic cleavage of peptide bonds are called proteases. Proteases fall into four main mechanistic classes: serine, cysteine, aspartyl and metalloproteases. In the active sites of seri...
The primary subsite specificities of human leukocyte elastase, cathepsin G, porcine pancreatic elastase, rat mast cell proteases I and II, bovine chymotrypsin A alpha, and the protease from strain V-8 of Staphylococcus aureus have been mapped w...
1999. Dengue virus NS3 serine protease: crystal structure and insights into interaction of the active site with substrates by molecular modeling and structural analysis of muta- tional effects. J. Biol. Chem. 274:5573-5580.Krishna Murthy HM, Clum S and Padmanabhan R. Dengue virus NS3 seri...
The role of the serine protease active site in the mode of action of epidermolytic toxin of Staphylococcus aureus The sequences of the epidermolytic toxins and V8 serine proteinase share about 25% identity, including the catalytic triad at the proteinase active centre... MB Redpath,TJ Foster,CJ ...
serine protease inhibitioninhibition by somanirreversible inhibitionThe stereoselectivity of phosphonylation of serine hydrolases by the ROR'P(O)X group of compounds is governed by the electronic properties of X and the size of RO. The electronic properties of ligands attached to P are decisive in ...
X-ray snapshots of serine protease catalysis reveal a tetrahedral intermediate. Nat Struct Biol 8 : 689-694.Wilmouth RC., Edman K., Neutze R., Wright PA., Clifton IJ., Schneider TR., Schofield CJ., Hajdu J. (2001). X-ray snapshots of serine protease catalysis reveal a tetrahedral ...
These findings are of general interest when the affinities and specificities of serine protease inhibitors to be used for pharmacological intervention are considered and could pave the way for potential drug candidates for the treatment of cancer. 展开 关键词: plasminogen-activator species-specificity ...
3c); (ii) for Vpr fibrils, the ModelAngelo model sequences were used to search for proteins from all organisms, and Vpr from Bacillus amyloiquefaciens were the top hits; (iii) the final model for Vpr contains a well folded catalytic triad typical for serine protease (Fig. 2a, b). In...