In this process, the strand folds over, and the adapter region hybridizes to the 2nd type of oligo on the flow cell.20 00:01:38,060 --> 00:01:42,626 Polymerases generate the complementary strand, forming a double stranded bridge. 21 00:01:42,626 --> 00:01:49,360 This bridge i...
and the prepared libraries are cleaned, quantitated, and normalized before loading onto a flow cell (Figure 1). Since library preparation plays a critical role in obtaining high-quality data [1], researchers should understand the ...
别名: Illumina高通量DNA测序 Illumina高通量DNA测序(Illumina HT DNA Sequencing Service)服务介绍本公司跟国内外相关单位合作推出的Illumina高通量DNA测序服务是基于美国Illumina公司的Genome Analyzer IIx测序仪开展的测序服务,该仪器是目前广泛使用的高通量DNA测序仪之一,它具有下列特点:1. 基于Sequencing-by-synthesis技...
3.1.3 Sequencing by Synthesis Illumina sequencing is done simultaneously for each and every cluster on a flow cell. The sequence of the genomic DNA fragments are determined by supplying a sequencing primer, DNA polymerase, and fluorescently-labeled nucleotides with each base connected to a different...
Sequence-by-synthesis A sequencing technology used primarily by Illumina, in which a DNA polymerase synthesizes a strand of DNA complementary to a template by incorporating a fluorescently labelled deoxynucleoside triphosphate that is imaged to identify the base and then cleaved before the process is ...
This protocol details the implementation of Seq-Scope with an Illumina NovaSeq 6000 sequencing flow cell, allowing the profiling of multiple tissue sections in an area of 7 mm 脳 7 mm or larger. We describe the preparation of a fresh-frozen tissue section for both histological imaging and ...
J. Low-bias, strand-specific transcriptome Illumina sequencing by on-flowcell reverse transcription (FRT-seq). Nat. Protoc. 6, 1736–1747 (2011). CAS PubMed Google Scholar Koch, C. et al. Nanopore sequencing of DNA-barcoded probes for highly multiplexed detection of microRNA, proteins and...
The Illuminanext-generation sequencing (NGS)method is based on sequencing-by-synthesis (SBS), and reversible dye-terminators that enable the identification of single bases as they are introduced into DNA strands. Theoretically, Illumina NGS technology resemblesSanger sequencing. During the cyclic process...
33、This stepuses theresynthesis mix duringthe Index 2 Readprocess. Document#15057455 v03 For Research Use Only. Not for use in diagnostic procedures. 6 IndexedSequencing OverviewGuide Figure 4 Dual-Indexed Sequencing on a Single-Read Flow Cell (HiSeq 4000 or HiSeq 3000) 1Read1Read1Read 1fo...
SYBR Green dye was added to all flowcell lanes, and a portion of each lane was visualized using a light microscope, to confirm target cluster density. Flowcells were sequenced on HiSeq 2000 using HiSeq 2000 v2 or v3 Sequencing-by-Synthesis kits, then analysed using RTA v1.10.15. or RTA...