Historically, the prospect of developing mRNA vaccines was uncertain due to concerns of mRNA instability and the feasibility of large-scale manufacturing. Today, these issues are no longer perceived as barriers in the widespread implementation of the technology. Currently, nonamplifying mRNA vaccines ...
It references the article "Nonviral delivery of self-amplifying RNA vaccines," by A. Geall and colleagues in the 2012 issue of the "Proceedings of the National Academy of Sciences" (PNAS). Moreover, it was found that injection of mRNA or plasmid DNA (pDNA) into a mice's skeletal ...
Self-Amplifying RNA Viruses as RNA Vaccines. Int. J. Mol. Sci. 2020, 21, 5130. [Google Scholar] [CrossRef] Moyo, N.; Vogel, A.B.; Buus, S.; Erbar, S.; Wee, E.G.; Sahin, U. Efficient Induction of T Cells against Conserved HIV-1 Regions by Mosaic Vaccines Delivered as Self...
Vaccinology is shifting toward synthetic RNA platforms which allow for rapid, scalable, and cell-free manufacturing of prophylactic and therapeutic vaccines. The simple development pipeline is based on in vitro transcription of antigen-encoding sequences
RNA-based vaccines are an attractive candidate for this role: they are safe, are produced cell free, and can be rapidly generated in response to pathogen emergence. Two RNA vaccine platforms are available: synthetic mRNA molecules encoding only the antigen of interest and self-amplifying RNA (sa...
Self-amplifying vaccines promise extended efficacy with lower doses than their mRNA predecessors, but approvals have been slow to materialize. Credit: Javier Zayas Photography/ Getty Images A year after bursting onto the scene amid the RNA renaissance, scientific challenges and regulatory resistance...
“As for all self-amplifying vaccines, concerns have been raised over adverse events in vulnerable individuals. For example, replicon [sa-mRNA] vaccines could persist in immunocompromised individuals as clearance may be less efficient,” it said. ...
We report on a highly sensitive lateral flow immunoassay (LFIA) for influenza A which serves as a model antigen. Gold nanoparticles conjugated to monoclona... N Wiriyachaiporn,W Maneeprakorn,C Apiwat,... - 《Microchimica Acta》 被引量: 22发表: 2015年 Self-Amplifying mRNA Vaccines Expressing...
Placental Ischemia-Induced Hypertension in the Rat.###Leukoreduction for the prevention of adverse reactions from allogeneic blood transfusion.###Induction of Broad-Based Immunity and Protective Efficacy by Self-amplifying mRNA Vaccines Encoding Influenza Virus Hemagglutinin.###Interleukin-6 and C-react...
However, limitations in these vaccines have been recognized due to their requirement for cold storage, short durability of protection, and lack of access in low-resource regions. We have developed an easily-manufactured, potent self- amplifying RNA (saRNA) vaccine against SARS-CoV-2 that is ...