We systemically investigated the effect of monocyte (MO) seeding density on the generation of monocyte-derived immature DCs (iDCs) in MicroDEN, a perfusion-based culture system, as well as 6-well plates. Cell surface markers and the ability of the iDCs to induce proliferation of allogeneic T ...
After counting the cells, a number of cells equal to the initial seeding density of their respective group were subcultured to a new well. The cells were cultured at 37 °C in a humidified atmosphere containing 5% CO2 for 28 days. In all the experiments, 24-well culture plates ...
BS cells were plated in poly-L-lysine treated 6 well plates at a density of 100’000 cells/well. Cells were allowed to grow and divide in an incubator at 37 °C. BS cultures were allowed to reach a confluency of 50–60%. The ClearTau sonicated fibril seeds were incubated with ...
Methods and materials αS FRET seeding assay Generally, HEK 293T αS-CFP/YFP is plated in a black- bottomed 96-well plate with the density of 80 k/well in DMEM media with 10% FBS and Penicillin-Streptomy- cin. Three control cell lines – no-transfected HEK 293T cells, αS-...
The dissociated neurons were plated in 6-well plates, onto coverslips (VWR) or in clear black-bottom 96-well plates (Falcon) freshly coated with poly-l-lysine 0.1% wt/vol in water (Brunschwig). Neurons were plated at a density of 300,000 cells per ml for biochemistry analyses, 250,...
Cell culture media tend to foam due to the high protein content of supplemented serum. Air bubbles can hinder cell attachment during seeding and therefore create well-to-well variations of cell numbers (Image 2). Especially in smaller plate formats like...
Inhibiting fibril growth attenuatesα-syn-induced toxicity in hippocampal primary neurons. Hippocampal primary culture were plated in 96-well plates and treated for 6 days with Tris buffer (negative control) or (a) the indicatedα-syn species in the presence of DMSO (negative control) or Tolcapone...
The day prior to transduction, we used a 4-min incubation with Accutase to detach hiPS cells and subsequently counted and seeded cells onto Geltrex-coated 6-well plates at a density of 1 × 105 cells per well in iPS-Brew + 2μM Thiazovivin. The next day, to transduce hiPS cells, we...
Richter transformation (RT) is a paradigmatic evolution of chronic lymphocytic leukemia (CLL) into a very aggressive large B cell lymphoma conferring a dismal prognosis. The mechanisms driving RT remain largely unknown. We characterized the whole genome,
The neurons were seeded in 96-well plates or onto coverslips (CS; VWR) previously coated with poly-L-lysine 0.1% w/v in water (Sigma-Aldrich) at a density of 150 000 cells/ml. After 7 days, the cells were treated with cytosine β-D-arabinofuranoside (Sigma-Aldrich) to a final ...