To assess the effect of B:C ratio on T cell expansion, PBMCs were seeded at a concentration of 250 × 103 cells/mL in a 24-well culture plate (based on the results of seeding density comparative groups), and were then stimulated once with microbeads at different B:C ratios (1:10...
Interestingly, the cells growing in wells containing TCPL disks showed slightly more cell numbers than cells growing on plastic alone for each density. The results also revealed no significant differences between the groups for parameters of cell function such as protein concentration and markers of ...
FRET density was normalized to the amount of total tau present in the sample. Data are presented as mean ± SD (***p < 0.001; ****p < 0.0001). d A representative electron microscopy (EM) image showing straight tau filaments in the sarkosyl-insoluble GGT-tau fraction Full ...
Another explanation may be related to the physiologically higher innervation density in proximal vs distal skin29, with consequently higher amount of tau and higher propensity to seed/aggregate. This might also explain the lower aggregation activity in PD in cervical compared to MSA and HC; indeed,...
Richter transformation (RT) is a paradigmatic evolution of chronic lymphocytic leukemia (CLL) into a very aggressive large B cell lymphoma conferring a dismal prognosis. The mechanisms driving RT remain largely unknown. We characterized the whole genome,
Transduction of biosensor cell lines, flow cytometry and seeding analyses The seeding assay was conducted as previously described [14] with the following changes: v2H cells were plated 20 h before seed transduction at a density of 16,000 cells/well in a 96-well plate in a media ...
Electron density in blue at a contour level of 1.0 σ displayed in the program Coot. In this initial map the electron density is nicely resolved and the model build into the electron density. The zoom in shows the residues His142 and Glu189, as well as a water molecule. Please click ...
To generate monoclonal cell lines from the polyclonal population, a limiting serial dilution protocol was used to seed individual cells in 96-well plates at an average density of 0.5 cells/well, and plates were kept in an incubator for 2 to 3 weeks. Genomic DNA was extracted from cells ...
The neurons were seeded in 96-well plates or onto coverslips (CS; VWR) previously coated with poly-L-lysine 0.1% w/v in water (Sigma-Aldrich) at a density of 150 000 cells/ml. After 7 days, the cells were treated with cytosine β-D-arabinofuranoside (Sigma-Aldrich) to a final ...
The dissociated neurons were plated in 6-well plates, onto coverslips (VWR) or in clear black-bottom 96-well plates (Falcon) freshly coated with poly-l-lysine 0.1% wt/vol in water (Brunschwig). Neurons were plated at a density of 300,000 cells per ml for biochemistry analyses, 250,...