The neurons were seeded in 96-well plates or onto coverslips (CS; VWR) previously coated with poly-L-lysine 0.1% w/v in water (Sigma-Aldrich) at a density of 150 000 cells/ml. After 7 days, the cells were treated with cytosine β-D-arabinofuranoside (Sigma-Aldrich) to a final ...
Fig. 2: Mutational processes in RT. a, Principal component analysis (PCA) of the 96-mutational profile of CLL and RT.b, Signatures identified de novo in CLL/RT not reported in COSMIC. The main peaks of each signature are labeled in black.c, Contribution of mutational processes in CLL/RT...
Furthermore, significant inhibitory effects on A7r5 proliferation were observed at both 5000 and 2500cellswell1for 72h. Data suggest that the effects of transient silencing on cell proliferation differ depending on the initial cell seeding density. While high seeding densities mask the significant ...
FRET density was normalized to the amount of total tau present in the sample. Data are presented as mean ± SD (***p < 0.001; ****p < 0.0001). d A representative electron microscopy (EM) image showing straight tau filaments in the sarkosyl-insoluble GGT-tau fraction Full ...
Does cell density matter? The uniformity of cell distribution can have a significant impact on your experiment. Of course, there are cell types that tend to grow in colonies rather than forming a homogenous monolayer. But in general, the cells should ...
To generate monoclonal cell lines from the polyclonal population, a limiting serial dilution protocol was used to seed individual cells in 96-well plates at an average density of 0.5 cells/well, and plates were kept in an incubator for 2 to 3 weeks. Genomic DNA was extracted from cells ...
The cells were plated in 96-well plates at an equal density (5 × 103). BrdU was added to the cells for 4 hrs, and the cells were treated according to the manufacture’s protocol. The optical density at 450 nm was measured using an ELISA plate reader. Apoptosis assay TUNEL assay...
To assess the effect of B:C ratio on T cell expansion, PBMCs were seeded at a concentration of 250 × 103 cells/mL in a 24-well culture plate (based on the results of seeding density comparative groups), and were then stimulated once with microbeads at different B:C ratios (1:10...
For whole cell lysate, bacteria grown on YESCA agar at 26 °C for 48 h were suspended in 50 mm potassium Pi, pH 7.2, normalized by optical density at 600 nm (A600), and pelleted down. The pellets were pre-treated with or without 70 μl of HFIP immediately followed by SpeedVac ...
Another explanation may be related to the physiologically higher innervation density in proximal vs distal skin29, with consequently higher amount of tau and higher propensity to seed/aggregate. This might also explain the lower aggregation activity in PD in cervical compared to MSA and HC; indeed,...