in 24-well plates (Falcon). All three species were cultured at both ~22 °C and 4 °C. The seeding density was 3.00E + 06 cells/mL forG. barretti, and 5.00E + 06 cells/mL forGeodiasp. andG. neptuni. Passaging was performed by resuspending the cells by pipetting...
A custom-made esiRNA library was purchased from Sigma/Eupheria Biotech (MISSION® esiRNA; Supplementary Table6). Cells were seeded into 96-well plates (flat-bottom, Costar) at a density of 7000 cells/well. Twenty-four hour after seeding, esiRNA was transfected in triplicates at a concentrati...
Cell culture media tend to foam due to the high protein content of supplemented serum. Air bubbles can hinder cell attachment during seeding and therefore create well-to-well variations of cell numbers (Image 2). Especially in smaller plate formats like...
Proliferation assay ARPE-19 e Y-negative clones 3, 5 and 9 were seeded in duplicates in 6 wells plates, 100.000 cells each well, and counted 24, 48 and 72 h after seeding. Growth curves were determined and reported in the chart. Experiments were repeated five times.Availability...
*Seeding density is given for each culture vessel type as follows: Dishes and Flasks: Cells per vessel; Culture plates: Cells per well. †The number of cells on a confluent plate, dish, or flask will vary with cell type. For this table, HeLa cells were used....
Isolated cells were seeded on human fibronectin (hFN)-coated 12-well plates at a density of 107 cells/well, cultured with EGM-2 in a 5% CO2 incubator at 37 °C. After 3 days in culture, non-adherent cells were discarded and the medium was changed every other day. Colonies of outgrowth...
20,000 sorted apCAFs from KPfC tumors were seeded in U-bottom 96-well plates and incubated with or without 25 μg/mL OVA peptide (OVA 323-339) (GenScript) in DMEM with 10% FBS for 4 h at 37°C. Spleens from 6-to-8-week-old OT II mice were harvested and CD4+ T cells were...
The cells were seeded on 96-well plates at a cell density of 1×104 cells and cultured for 7 days with medium replacement on culture day 3. On culture day 7, AlamarBlue® reagent (Thermo Fisher Scientific) was added into each test well and after three-hour incubation in CO2 incubator ...
Extreme limiting dilution assay was performed as described [63,64]. Different dilutions (1, 10, 25, 50 cells/well) for HCT 116 cells were seeded in 96-well plates and treated with BSA or BSA-PA/OA (PA 100 µM, OA 200 µM) respectively. After 2 weeks, the number of sphere...
Optimizing interleukin-2 concentration, seeding density and bead-to-cell ratio of T-cell expansion for adoptive immunotherapy Sasan Ghaffari Monireh Torabi-Rahvar Naser Ahmadbeigi BMC Immunology (2021) Characterization and classification of rat neural stem cells and differentiated cells by comparative...