e Interaction between purified SEC22B and GST-Flag-tagged STX18 using an in vitro GST pull-down assay followed by immunoblot (IB) and Coomassie blue staining. f Interaction between purified SNAP23 and GST-Flag-tagged STX18 using an in vitro GST pull-down assay followed by IB and Coomassie ...
pCMV-SPORT6-SEC22A是一个大肠杆菌表达载体,Tac强启动子可以驱动GST促溶标签和目的基因融合表达,LacI阻碍蛋白和Laco操作子使本质粒在没有加入IPTG之前禁止表达,防止其影响细菌生长。表达后的融合蛋白可用凝血酶蛋白酶切割,再过一次GST柱子即可清除掉GST标签。
Figure 3. Sec1p binds strongly to the t-SNARE complex (Sso1p/Sec9c) and the fully assembled ternary SNARE complex (Sso1p/Sec9c;Snc2p). GST pull-down experiments were used to assess the degree of Sec1p binding to various SNAREs and SNARE complexes. The level of nonspecific binding was de...
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(c) Total cellular GST activities were determined using the same cell lysates in (b) (n ¼ 3–4, ±S.E.M.). Significant differences between untreated Txnrd1fl/fl without additional selenite and the other samples are indicated (*Po0.05; n.s., not significant, P40.05) protective ...
9 Pin DSub 1/4 VCR SEC-4401M Untested AS-ISHoriba STEC SEC-Z524MGXN Mass Flow Controller AMAT 0190-33214 RefurbishedHoriba STEC SEC-V120DM Mass Flow Controller,V100 series,JapanHoriba STEC SEC-4400M SIH4 20 SCCM Mass Flow Controller MFCHORIBA STEC SEC-G100,SEC-G111AMC MISS FLOW CONTROLLER...
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实验结论:只有GST-H1片段结合Sec5蛋白,其它片段无明显结合。 (4)His标签融合人Sec5蛋白的构建 如图5所示,人Sec5蛋白全长有924个氨基酸,其中,人Sec5蛋白的氨基酸序列如SEQ ID NO:9所示,其中人Sec5蛋白-1片段、人Sec5蛋白-2片段、人Sec5蛋白-3片段和人Sec5蛋白-4片段的氨基酸序列如SEQ ID NO:10、SEQ ID ...
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pCMV-SPORT6-SEC14L4质粒结构是一个大肠杆菌表达载体,Tac强启动子可以驱动GST促溶标签和目的基因融合表达,LacI阻碍蛋白和Laco操作子使本质粒在没有加入IPTG之前禁止表达,防止其影响细菌生长。表达后的融合蛋白可用凝血酶蛋白酶切割,再过一次GST柱子即可清除掉GST标签。 产品相册pCMV-SPORT6-SEC14L4质粒结构TTTCCTGCGTT...