complex. Fig. 3 illustrates the results of a representative binding assay. Minimal amounts of Sec1p nonspecifically associated with reduced glutathione (GSH) resin (lane 1), GST (lane 2), or the Golgi SNARE Sed5p (lane 3). Specific Sec1p binding was detected to free GST-Sso1p (Fig. 3...
e Interaction between purified SEC22B and GST-Flag-tagged STX18 using an in vitro GST pull-down assay followed by immunoblot (IB) and Coomassie blue staining. f Interaction between purified SNAP23 and GST-Flag-tagged STX18 using an in vitro GST pull-down assay followed by IB and Coomassie ...
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pCMV-SPORT6-SEC23B质粒是一个大肠杆菌表达载体,Tac强启动子可以驱动GST促溶标签和目的基因融合表达,LacI阻碍蛋白和Laco操作子使本质粒在没有加入IPTG之前禁止表达,防止其影响细菌生长。表达后的融合蛋白可用凝血酶蛋白酶切割,再过一次GST柱子即可清除掉GST标签。 产品相册pCMV-SPORT6-SEC23B质粒TTTCCTGCGTTATCCCCTGATT...
gsten H ASM PN: 04-328301A05 KIT-EPROMS-EPI V4.02.14.02-MEM? Phoenix Contact MCR-C-UI-UI-DCI 3-Way Transmitter I Carten CB751-10 PC2FSM CU Tubes UHP Check Valve PN: Lam Research 676-090827-001 1/3 HP Pump Motor M&W C HP/Agilent 11730C Power Meter/SNS Noise 20ft Cable ASM ...
pCMV-SPORT6-SEC22A是一个大肠杆菌表达载体,Tac强启动子可以驱动GST促溶标签和目的基因融合表达,LacI阻碍蛋白和Laco操作子使本质粒在没有加入IPTG之前禁止表达,防止其影响细菌生长。表达后的融合蛋白可用凝血酶蛋白酶切割,再过一次GST柱子即可清除掉GST标签。
(c) Total cellular GST activities were determined using the same cell lysates in (b) (n ¼ 3–4, ±S.E.M.). Significant differences between untreated Txnrd1fl/fl without additional selenite and the other samples are indicated (*Po0.05; n.s., not significant, P40.05) protective ...
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We also show that active forms of ARF4 and ARF5 co-precipitate with BIG2 and that this interaction is mediated by the N-terminal region of BIG2, the same region that is sufficient to recruit BIG2 to TGN membranes in vivo (9). Thus, GBF1 appears to act as a master regulator of ...