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f Interaction between purified SNAP23 and GST-Flag-tagged STX18 using an in vitro GST pull-down assay followed by IB and Coomassie blue staining. g Interaction between purified SEC22B and GST-Flag-tagged SNAP23 using an in vitro GST pull-down assay followed by IB and Coomassie blue staining...
4: ASM PN: 16-321908C01 Lamp-Linear-Tun?gsten H ASM PN: 04-328301A05 KIT-EPROMS-EPI V4.02.14.02-MEM? Phoenix Contact MCR-C-UI-UI-DCI 3-Way Transmitter I Carten CB751-10 PC2FSM CU Tubes UHP Check Valve PN: Lam Research 676-090827-001 1/3 HP Pump Motor M&W C HP/Agilent 11...
(c) Total cellular GST activities were determined using the same cell lysates in (b) (n ¼ 3–4, ±S.E.M.). Significant differences between untreated Txnrd1fl/fl without additional selenite and the other samples are indicated (*Po0.05; n.s., not significant, P40.05) protective ...
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pCMV-SPORT6-SEC14L4质粒结构是一个大肠杆菌表达载体,Tac强启动子可以驱动GST促溶标签和目的基因融合表达,LacI阻碍蛋白和Laco操作子使本质粒在没有加入IPTG之前禁止表达,防止其影响细菌生长。表达后的融合蛋白可用凝血酶蛋白酶切割,再过一次GST柱子即可清除掉GST标签。 产品相册pCMV-SPORT6-SEC14L4质粒结构TTTCCTGCGTT...
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lane 4 vs. lane 3), but not to free GST-Sec9c (Fig. 3, lane 7). Binding of Sec1p to the t-SNARE complex (Fig. 3, lanes 5 and 8) was 4–10-fold more than background values. Significant binding of Sec1p to the ternary SNARE complex (Fig. 3, lanes 6 and 9) was also obs...
pCMV-SPORT6-SEC22A是一个大肠杆菌表达载体,Tac强启动子可以驱动GST促溶标签和目的基因融合表达,LacI阻碍蛋白和Laco操作子使本质粒在没有加入IPTG之前禁止表达,防止其影响细菌生长。表达后的融合蛋白可用凝血酶蛋白酶切割,再过一次GST柱子即可清除掉GST标签。
We also show that active forms of ARF4 and ARF5 co-precipitate with BIG2 and that this interaction is mediated by the N-terminal region of BIG2, the same region that is sufficient to recruit BIG2 to TGN membranes in vivo (9). Thus, GBF1 appears to act as a master regulator of ...