Among these, the thioredoxin (Trx)- and glutathione (GSH)-dependent systems together with specia- lized enzymes such as superoxide dismutases and catalase may act in concert.4,5 Based upon the results of the present study, we suggest that the Trx system is absolutely required for protection ...
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e Interaction between purified SEC22B and GST-Flag-tagged STX18 using an in vitro GST pull-down assay followed by immunoblot (IB) and Coomassie blue staining. f Interaction between purified SNAP23 and GST-Flag-tagged STX18 using an in vitro GST pull-down assay followed by IB and Coomassie ...
gsten H ASM PN: 04-328301A05 KIT-EPROMS-EPI V4.02.14.02-MEM? Phoenix Contact MCR-C-UI-UI-DCI 3-Way Transmitter I Carten CB751-10 PC2FSM CU Tubes UHP Check Valve PN: Lam Research 676-090827-001 1/3 HP Pump Motor M&W C HP/Agilent 11730C Power Meter/SNS Noise 20ft Cable ASM ...
but not to free GST-Sec9c (Fig. 3, lane 7). Binding of Sec1p to the t-SNARE complex (Fig. 3, lanes 5 and 8) was 4–10-fold more than background values. Significant binding of Sec1p to the ternary SNARE complex (Fig. 3, lanes 6 and 9) was also observed, strengthening a pre...
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pCMV-SPORT6-SEC22A是一个大肠杆菌表达载体,Tac强启动子可以驱动GST促溶标签和目的基因融合表达,LacI阻碍蛋白和Laco操作子使本质粒在没有加入IPTG之前禁止表达,防止其影响细菌生长。表达后的融合蛋白可用凝血酶蛋白酶切割,再过一次GST柱子即可清除掉GST标签。
实验结论:只有GST-H1片段结合Sec5蛋白,其它片段无明显结合。 (4)His标签融合人Sec5蛋白的构建 如图5所示,人Sec5蛋白全长有924个氨基酸,其中,人Sec5蛋白的氨基酸序列如SEQ ID NO:9所示,其中人Sec5蛋白-1片段、人Sec5蛋白-2片段、人Sec5蛋白-3片段和人Sec5蛋白-4片段的氨基酸序列如SEQ ID NO:10、SEQ ID ...
We also show that active forms of ARF4 and ARF5 co-precipitate with BIG2 and that this interaction is mediated by the N-terminal region of BIG2, the same region that is sufficient to recruit BIG2 to TGN membranes in vivo (9). Thus, GBF1 appears to act as a master regulator of ...