we sequenced 892 SARS-CoV-2 genomes collected from patients in Saudi Arabia from March to August 2020. We show that two consecutive mutations (R203K/G204R) in the nucleocapsid (N) protein are associated with higher viral loads in COVID-19 patients. Our ...
There is almost no report of SARS-CoV-2 RNA polyadenylation up to date. The newly synthesized SARS-CoV-2 +gRNA has a ~ 30–60-nt-long (47 nts in median length) poly-A tail on its 3' end [35]. Since hCoV RNA genomes don’t have a conventional poly-A signal and are transcribe...
Unsupervised cluster analysis of SARS-CoV-2 genomes reflects its geographic progression and identifies distinct genetic subgroups of SARS-CoV-2 virusSARS-CoV-2clusteringcovidjaccardOver 10,000 viral genome sequences of the SARS-CoV-2virus have been made readily available during the ongoing coronavirus...
mutation in the S protein that may increase the SARS-CoV-2 infectivity [119]. Similarly, three deletions have been reported in the genomes of SARS-CoV-2 from Japan (Aichi), the USA (Wisconsin), and Australia (Victoria) [120]. Two of these deletions (3 nt and 24 nt) were in the OR...
2.2.1.2 在跳出来的输入结果中点击“Genomes”部分的“Genome”项,点击右侧数字一进入; 2.2.1.3 在网页下面“Representative(genome information for reference and representative genomes)”中的RefSeq栏中有编号 NC_045512.2,点击编号链接进入Wuhan-Hu-1序列的Genbank格式页面; ...
原文检索: Irwin Jungreis, Rachel Sealfon, Manolis Kellis.SARS-CoV-2 gene content and COVID-19 mutation impact by comparing 44 Sarbecovirus genomes.Nature Communications, 2021; 12 (1) DOI:10.1038/s41467-021-22905-7
RNA duplexes usually are under selection pressure to maintain the viral genome’s conformation to facilitate its replication17. To uncover duplexes under purifying selection, we first conducted an analysis to examine the co-variant base pairs in 429 non-redundant coronavirus genomes. This comparison ...
The ARTIC panel consists of 98 primers in two pools that amplify SARS-CoV-2 viral genomes. The sequencing data can elucidate the presence of mutations consistent with known variants or identify new variants in research studies. Learn more ...
“founder effect” occurs when a limited number of individual viruses establish a new population during transmission. The mutations present in the genomes of these viral ancestors will dominate the population regardless of their effects on viral fitness. This same interplay of natural selection and ...
Coronavirus genomes consist of single-stranded, positive-sense RNA and are the largest among all known RNA viruses (27–32 kb)5. Prior to the emergence of SARS-CoV-2, most studies on secondary structures within coronavirus RNA genomes focused on several conserved regions that are essential for...