GeneXpert作为一种快速、简单、去中心化的分子POCT检测技术,能够突破现有检测技术对人员和场地的限制,实现疑似患者的快速诊断和密切接触人群的快速筛查。 荧光热对流PCR(C-PCR)是近年来发展起来的一种新的核酸检测技术,其利用加热形成温度梯度,造成密度差之后产生对流,使变性、退火、延伸等3个步骤合而为一,在反应管的...
[5]Yin, S., et al., The cryptic unstable transcripts are associated with developmentally regulated gene expression in blood-stage Plasmodium falciparum. RNA Biol, 2020. 17(6): p. 828-842. 编辑︱小满本 文完
[36] Hu B, Zeng LP, Yang XL, et al. Discovery of a rich gene pool of bat SARS related coronaviruses provides new insights into the origin of SARS coronavirus[J] PLoS Pathog, 2017, 13(11), https://doi.org/10.1371/journal.ppat.1006698. [37] John, Stavrinides, David S, Guttman. ...
GeneArt 基因合成与基因到蛋白质的服务参见thermofisher.com/geneart 用于流行病学研究的 cDNA 合成 开发了 Invitrogen SuperScript 逆转录酶,以改善热稳定性、持续合成能力和 cDNA 得率,目的是即使在使用极具挑战性的 RNA 样本时也能实现卓越的性能。迄今为止,SuperScript 逆转录酶的引用、...
SARS-CoV-2 receptor ACE2 is an interferon-stimulated gene in human airway epithelial cells and is enriched in specific cell subsets across tissues. Cell 181, 1016–1035 (2020). 11. Souyris M., et al. TLR7 escapes X chromosome inactivation in immune cells. Sci. Immunol. 3, eaap8855 (...
Gene networks of SARS-CoV-2 targeted human proteins expressed in testis (a) and ovary (b) and their interactions with the list of genes of our interest and gonadal steroid hormones. The PPI network constructed by GeneMANIA shows the interconnection between SARS-CoV-2 targeted human proteins expr...
相关研究结果以论文手稿的形式在线发表在Cell期刊上,论文标题为“SARS-CoV-2 receptor ACE2 is an interferon-stimulated gene in human airway epithelial cells and is detected in specific cell subsets across tissues”。 图片来自Cell, 2020, doi:10.1016/j.cell.2020.04.035。
4)通过扩增n端spikegene,来检测病人外周血和尿液中的新冠病毒sars-cov-2,是不稳定可靠的。 ngs方法在新冠病毒sars-cov-2的检测和鉴定中发挥了不可替代的作用,相比广泛应用的rt-pcr法核酸检测技术,准确率和敏感性更高。所以,《新型冠状病毒肺炎诊疗方案(试行第七版)》,增加“采用rt-pcr或/和ngs方法”进行核酸检...
Ziegler, C.G.K., Allon, S.J., Nyquist, S.K., et al. (2020). SARS-CoV-2 receptor ACE2 is an interferon-stimulated gene in human airway epithelial cells and is detected in specific cell subsets across tissues. Cell 181, 1016-1035.e19....
Although different from SARS-CoV and other hCoVs in numbers of encoded accessory proteins and lacking a hemagglutinin esterase (HE) gene found in hCoV-OC43 and hCoV-HKU1 (Fig.1), the SARS-CoV-2 genome has the coding capacity and strategies for nonstructural proteins (nsps) and structural pr...