作者还发现在肺部的不同细胞类型中，髓细胞（Myeloid cells），特别是CD14highCD16high炎性单核细胞和LDB2highOSMRhighYAP1high巨噬细胞，含有更多的SARS-CoV-2病毒RNA。值得注意的是，这些表达SARS-CoV-2病毒RNA的细胞并不同时表达ACE2，TMPRSS2等病毒进入因子。除单细胞（核）图谱外，本文还采用NanoString GeoMx...
Coronavirus sickness (COVID-19) is associate degree communicable disease caused by a completely unique severe acute metabolic process syndrome coronavirus two (SARS-CoV-2) that originally started in metropolis province in China and has currently affected >200 countries worldwide and declared a plague[...
由于SARS-CoV-1和SARS-CoV-2之间的高度同源性,它们共享一些抗原区域。类似地,MERS-CoV和SARS-CoV-2在S糖蛋白上有35%的同源性(32)。最重要的是,来自SARS-CoV-1和SARS-CoV-2的S糖蛋白的RBD具有高度同源性,并且两者都参与与ACE2...
The UCSC SARS-CoV-2 Genome Browser (https://genome.ucsc.edu/covid19.html) is an adaptation of our popular genome-browser visualization tool for this virus, containing many annotation tracks and new features, including conservation with similar viruses, immune epitopes, RT–PCR and sequencing ...
本研究也和前面几项研究共同表明,SARS-CoV-2的ADE抗体有至少两种机制:RBD特异性的ADE抗体依赖于Fc-FcγRII;NTD特异性的ADE抗体则不依赖Fc,而是通过改变S蛋白构象进而影响S蛋白与受体ACE2的结合。 重要的是,无论RBD特异性的ADE抗体(DH1041)和NTD特异性的ADE抗体(DH1052),在小鼠和猴子中未表现出明显的影响如...
新冠肺炎由SARS-CoV-2病毒引发,其感染程度从无症状感染,到重症新冠肺炎,乃至死亡不等。导致病人死亡的首要因素为急性肺部损伤与急性呼吸窘迫综合征,以及多器官衰竭的并发症。而此外,重症新冠肺炎通常伴随着不恰当的促炎症宿主免疫反应和减弱的抗病毒...
SARS-CoV-2 genome structure and expression Like other hCoVs, SARS-CoV-2 has a single-stranded, positive-sense RNA (+RNA) genome of 29,882 [31], 29,891 [4] or 29,903 nucleotides (nts) [5]. The genome is packed by viral nucleocapsid (N) proteins as a large ribonucleoprotein (RNP)...
Fig. 1: Bidirectional CRISPR screens identify host factors critical for SARS-CoV-2-mediated CPE. a, Schematic of genome-wide CRISPR KO and activation screens for SARS-CoV-2 host factors, conducted in parallel. Calu-3 cells stably expressing Cas9 for the LOF screen or dCas9 and transcriptional...
手册中主要介绍了对SARS-CoV-2基因组序列的处理和分析,序列可以是从公共数据库下载或者是本地生成的数据。每个单独基因组序列被分成一系列子序列(从序列中提取多个部分序列),每一个序列会根据参考基因组进行SNP的分析。所有的SNP以开放的(动态)字符集存储,这些字符集
Arising mutations of the SARS-CoV-2 genome calls for global due diligence to closely monitor any variants to ensure diagnostic tests, vaccines, antiviral therapies and public health control measures remain effective. In order to track variants, a portion of positive SARS-CoV-2 samples should be ...