Expression and functional activity of ClyA and FlaB were assessed by performing western blotting, quantitative RT-PCR, hemolysis test, and the NF-κB binding assay. The therapeutic efficacy of the engineered bacteria was evaluated in mice bearing primary or metastatic tumors of mouse and human ...
Obovatol had observable effect on the TTSS-mediated contact-dependent hemolysis of sheep red blood cells. S. typhimurium SL1344 was incubated with sheep blood cells for 2 h in the absence or presence of various concentrations of obovatol and hemolytic activity was measured as the absorbance at...
Specific nanobodies developed against the Shigella invasion plasmid antigen D (IpaD) were found to reduce Shigella infection, and modified VHH heterodimers could reduce hemolysis induced by Shigella by 80% [17]. Nanobodies against Vibrio cholerae lipopolysaccharides were effective in neutralizing V. ...
the cultures were streaked onto Mueller Hinton agar surface (Difco, Michigan, USA) previously supplemented with 5% (v/v) sheep blood (Ebefarma, Rio de Janeiro, Brazil). The plates were incubated at 37 °C under
The number of living bacteria was counted by plating from serial dilutions of cultures on SS agar. The number of Salmonella was 2 × 109 CFU per ml for the CL-55-treated cultures and control. We did not find a significant difference in the rate of bacteria growth between the CL-55-...
single treatment with 12.5 μg of SM per larva. Larva death was monitored every 12 hrs for three days after treatment. Both untreated larvae (NC) and larvae treated with 1% dimethyl sulfoxide (DMSO) had 100% survival (blue line). RBCs: red blood cells; n = 15 replicates per ...
Overnight-grown bacterial cultures were streaked on Blood agar (Oxoid, Hampshire, UK). Agar plates were incubated at 37 °C for 48–72 h and were observed for the formation of any clean (α-hemolysis), greenish (β-hemolysis) hemolytic zones or no zone (γ-hemolysis) around the LAB ...
Blood samples were collected into sterile tubes with clot activator and gel for serum separation. The samples were then stored at room temperature (RT) for 30 min. After that, the samples were centrifuged for 5 min at 3000 rpm. Only the serum samples without hemolysis and lipemia were used...
(Poly)phenols on Metabolic Pathways and Functional Biomarkers: From Preclinical Models to Human StudiesEssential OilsEssential Oils: Chemistry and Food ApplicationsFactors Affecting Food Preferences and Their Association with ConsumptionFermented Foods and ProbioticsFinal Meeting of the GourMed PRIMA Project: ...