(perhaps the water condensate) on the agar surface. These strains sharedantigenic propertiestermed H-antigens (Hauch: breath). Later it was recognized that this diffuse colony morphology results from the motility of the individual organisms and that the H-antigens are associated with the flagella ...
Briefly, 1 μl of bacterial supernatant was spotted onto a blood agar plate and incubated overnight at 37 °C. Areas of blood cell lysis appeared as spots on the agar. The activity of FlaB secreted from the transformed SAM was measured in human TLR5 (hTLR5)-transfected cells after ...
For experiments conducted under traditional laboratory conditions, an overnight culture from a single colony of STY or STM was prepared in Miller formula LB-broth (Sigma, St. Louis, MO) at 37 °C with shaking at 225 rpm. The next day, overnight cultures were diluted 1:50 into ...
Morphology and infective infiltration ability of the strains After obtaining LNT MACS/VNP cells, the cells were lysed using 0.5% Triton X-100 to release the intracellular strain. The strains (released VNP) in the supernatant were collected and cultured for 6 h in LB liquid medium for amplific...
After incubation, at 37 °C for 24, 48 and 72 hrs subcultures were done on MacConkey agar and blood agar and were observed for bacterial growth after 24 hrs of aerobic incubation at 37 °C. Isolates were identi- fied by biotyping (colony morphology, staining reaction and biochemical ...
morphology from a BALB/c background16, we used a murine colon carcinoma CT26 cell line with fibroblast morphology from a BALB/c background17to represent different cancer types from the same background, which showed similar inhibitory effects on lung metastasis upon YB1 treatment (Fig.1f). ...
For invasion assays, cells were seeded on 24-well plates at a density of 5 × 104 cells per well, and grown for three days (changing media every other day). The day before the assay, a single colony of each bacterial strain was inoculated in 3 ml of LB broth and grown overnight in...
Burkholder KM, Thompson KL, Einstein ME, Applegate TJ, Patterson JA: Influence of stressors on normal intestinal microbiota, intestinal morphology, and susceptibility to Salmonella Enteritidis colonization in broilers. Poult Sci. 2008, 87 (9): 1734-1741. 10.3382/ps.2008-00107. Article CAS PubMed...
(Agilent, Santa Clara, CA, USA). Additionally, bacterial concentration was confirmed by serial dilution and determination of colony-forming units (CFU) on Mueller-Hinton agar plates (Sigma-Aldrich, St. Louis, Missouri, USA). Bacteria were diluted in serum- and antibiotic-free culture medium to...
Initially, 17 isolates from 10 poultry fecal samples were isolated based on their colony morphology, Gram staining, and biochemical characteristics. However, only three strains, L. reuteri PFS4, E. faecium PFS13, and E. faecium PFS14, were retained based on the selection criteria. The findings...