Pure single colonies were picked from BHI agar, inoculated in 100 µl of sterile nuclease-free molecular grade water (Ambion, USA) using a sterile disposable plastic loop and placed on a heated thermal block at 95°C for 10 min in micro centrifuge tubes for cell lysis. The tubes were ...
Mice were infected by oral gavage with 50 μl of bacterial suspension and inoculum was enumerated by plating on selective MacConkey agar plates. Growth of C. rodentium (Schauer and Falkow, 1993) was performed in a similar way, but plain LB was used for overnight as well as subculture. ASF...
To evaluate the protective effect of AP2 against ST infection in vivo, C57BL/6 mice were pre-treated with or without AP2 prior to ST challenge. Mice in AP2 group maintained stable body weight, while those in ST group experienced significant weight loss on day 2. By day 3, both ST and...
monocytogenes strains onto brain heart infusion agar (Becton, Dickinson & Co., Sparks MD) supplemented with 50 µg/mL of nalidixic acid (BHI-N) and incubated at 35 ± 2°C for 24 h. A single colony from each Salmonella and L. monocytogenes strain was then transferred to 10 mL of ...
Colony-forming units were then determined by plating 100 μl aliquots of 10-fold serial dilutions on BHI-Agar plates. Bone marrow chimera 107 bone marrow cells from WT or FoxO3a−/− mice were injected into irradiated B6 recipient mice, which were then maintained on antibiotics and ...
Complete inactivation was confirmed by cultivating an aliquot of prepared vaccine in BHI agar at 37 °C for 48 h. (Vaccine and Reagent Preparation Laboratory of the “Experimental Zooprophylactic Institute of Lombardy and Emilia Romagna”, IZSLER). S. Typhimurium ΔznuABC and virulent S. ...
1996). Five ml of 16–18 hours growth of positive isolates in brain heart infusion (BHI) broth was streaked on Mueller–Hinton agar (Himedia) plates by pour plate method. Sixteen antibiotic discs (Himedia) namely amikacin (30 mcg), ampicillin (10 mcg), ampicillin–salbactum (10 mcg), ...
After administration, the remainder of the inoculum cultures was transported to the microbiological laboratory on melting ice for plate counts on sheep-blood agar (incubated as above).A.H. HavelaarJ. GarssenK. TakumiM.A. KoedamJ.B. Dufrenne...
Calcofluor has previously been used to detect cellulose-producing strains ofS. Typhimurium as this dye binds polysaccharides with 1,4β-glucopyranosyl units and fluoresces under UV light [24] To assess cellulose production, we grew each strain on CFA Calcofluor agar at 25 and 37°C and compared...
In the event that no growth occurred on the MacConkey Agar/nalidixic acid plates, the backup wash was examined for the presence of salmonellae using FDA BAM procedures. The results achieved are shown in Table II which follows. The data for storage at 2.2° C. (36° F.) for 0, 5 ...