The culturability loss was monitored in the brain heart infusion (BHI) broth and agar, and the viability was determined by fluorescence microscopy, using the Live/Dead kit, and by flow cytometry. Besides, the morphological characterization by atomic force microscopy (AFM) was performed. Storage ...
1996). Five ml of 16–18 hours growth of positive isolates in brain heart infusion (BHI) broth was streaked on Mueller–Hinton agar (Himedia) plates by pour plate method. Sixteen antibiotic discs (Himedia) namely amikacin (30 mcg), ampicillin (10 mcg), ampicillin–salbactum (10 mcg), ...
Complete inactivation was confirmed by cultivating an aliquot of prepared vaccine in BHI agar at 37 °C for 48 h. (Vaccine and Reagent Preparation Laboratory of the “Experimental Zooprophylactic Institute of Lombardy and Emilia Romagna”, IZSLER). S. Typhimurium ΔznuABC and virulent S. ...
After administration, the remainder of the inoculum cultures was transported to the microbiological laboratory on melting ice for plate counts on sheep-blood agar (incubated as above).A.H. HavelaarJ. GarssenK. TakumiM.A. KoedamJ.B. Dufrenne...
typhimurium SL1344 was inoculated onto the surface of the agar. The plates were incubated at 37 °C for 20 h and motility was measured as the diameter of the halo zone. Bacterial growth S. typhimurium SL1344 was inoculated into BHI broth and cultivated for 14 h at 25 °C. Then, these...
1994, 721 strains of 75 different serovars isolated in 1995, 1,219 strains of 83 different serovars isolated in 1996, and 1,336 Salmonella strains of 92 different serovars isolated in 1997, for resistance to 17 antibiotics at one to three different concentrations with the agar dilution method...
The potential inhibitory effect of CFS-A samples againstS. Heidelberg IOC 969/17 was investigated by the agar diffusion method, specifically spot-on-the-lawn methodology42. For that, the bioindicator strain was precultivated at 37 °C in BHI broth and after 18 h it was 100-fold diluted...
were enriched in the BHI broth and moved up to selective agar medium in the pipettes. They changed the color of the agar and also caused turbidity of the BHI on top of the selective agar. Three treatments were used to evaluate the MP method; (1) L. monocytogenes (103–4 CFU/mL), (...
were enriched in the BHI broth and moved up to selective agar medium in the pipettes. They changed the color of the agar and also caused turbidity of the BHI on top of the selective agar. Three treatments were used to evaluate the MP method; (1) L. monocytogenes (103–4 CFU/mL), (...
During storage of PJ at 4?°C for 70 days, numbers of non-injured and sub-lethally injured survivors were determined by plating diluted juice samples on selective and non-selective agar. The minimum inhibitory concentration (MIC) of isoeugenol for E. coli O157:H7 and S. enterica was 0.5?