在两个独立的胶质瘤模型中,S100a4双阴性宿主小鼠明显比同一天移植相同胶质瘤细胞的B6小鼠存活时间长。还可以看到与B6相比,S100a4双阴性宿主小鼠CD3+,CD4+,CD8+T细胞的比例明显增加。 为了在功能上确定胶质瘤相关的S100a4双阴性免疫细胞是否更具有...
通过对肿瘤样本中超过200,000个细胞进行了单细胞RNA测序(single cell RNA sequencing,sc RNA-seq)和结果分析,发现肿瘤细胞的基因表达特点可以总结为四种不同分子亚型:OPC-like细胞,NPC-like细胞,AC-like细胞和Mes-like细胞,其中Mes-like细胞与EMT,缺氧和IFNγ反应有关的通路富集密切相关.T淋巴细胞中PDCD1/PD1的...
CD4+ T cells were purified on the magnetic beads using the mouse CD4+ T cell isolation kit (StemCell Technology). The purity and viability of the isolated CD4+ T cells were 89% and 98%, respectively. The isolated CD4+ T cells were injected i.v. into the recipient mice (S100A4KO, n...
通过对肿瘤样本中超过200,000个细胞进行了单细胞RNA测序(single cell RNA sequencing,sc RNA-seq)和结果分析,发现肿瘤细胞的基因表达特点可以总结为四种不同分子亚型:OPC-like细胞,NPC-like细胞,AC-like细胞和Mes-like细胞,其中Mes-like细胞与EMT,缺氧和IFNγ反应有关的通路富集密切相关.T淋巴细胞中PDCD1/PD1的...
Latency-Reversing Agents Induce Differential Responses in Distinct Memory CD4 T Cell Subsets in Individuals on Antiretroviral Therapy Cell Reports, Volume 29, Issue 9, 2019, pp. 2783-2795.e5 Marion Pardons,…, Nicolas Chomont View PDF MLL1 Inhibition and Vitamin D Signaling Cooperate to Facilitate...
In addition, circulating CD4+ T cells and neutrophils (CD11b+Ly6g+Ly6cint) frequencies were also decreased in the 4T1 model. Others have shown that myeloid-derived suppressive cells (MDSC: CD11b+, Ly6gloLy6chi,IAIE-) play an important role in cancer metastasis. Our result suggests that ...
Using ELISA, confocal immunofluorescent microscopy, flow cytometry, and bulk RNA sequencing, we report that systemic administration of the S100A4-TfR BsA results in increased immune cell infiltration with a preferential increase in CD4 T cell infiltration, and potent immune remodeling of the tumor ...
The autocrine incursion of CD44ν6 by soluble OPN, can induce the loosening of cell-matrix adhesion and contributes to cell migration via Rho family GTPase activation. As matrix components, OPN and hyaluronate (HA) competitively interact with CD44; HA, but not OPN, induces standard CD44 (CD4...
To study mechanisms underlying differential effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and benzo(a)pyrene (B(a)P) on thymocyte differentiation, we examined effects of AhR ligands on the differentiation of \\{DPK\\} cells, a CD4+CD8+ thymic lymphoma cell line which can ...